Research Flow Cytometry Core

Software

The RFCC has a Mac and PC available in R5543 for data analysis. See below for a listing of software packages.
 

PC 

• FACSDiVa (BD Biosciences). Watch a video overview of FACSDiVa.
• FCSExpress (DeNovo Software). Visit DeNovo Software website for technical support.
• FlowJo (Tree Star Inc.). See the online manual for Windows.
• ModFit LT (Verity Software House). Watch video tutorials.
• FCAP Array (Soft Flow, Inc. ) for analysis of bead based multiplex assays (e.g., CBA). 

Mac 

• CellQuest Pro (BD Biosciences). Download the acquisition and analysis tutorials for more information.
• FlowJo (Tree Star Inc.). See the online manual for Mac.
   

NOTE: There are extra dongles for FACSDiVa and CellQuest Pro that can be signed out to use in individual labs. Please contact a member of the RFCC to sign out a dongle. When you return the dongle, make sure you have a member of the flow core sign it back in. If you sign out a dongle and misplace it or it is not returned, you will be charged for the replacement. 
 

FlowJo Site License

The RFCC hosts a site license for FlowJo. See below for more information and instructions for signing up.

Digestive Health Center members receive a discount. See the DHC website for more details. 

• Who can participate?
  Flow Core users from Cincinnati Children’s and UC can participate.
• How much is it?
  Based on current usage, it is around $250 / computer / year. The price per computer drops as the number of licenses increases. Please visit the pricing schedule for more information.
• What if I already have FlowJo?
  If you currently have FlowJo, you may turn in your dongle for a $1,000 credit towards the site license. After that you will pay for each seat.
• How do I sign up? 
1. Fill out the license agreement and return it to the Flow Core. We will give you a username and password for the registration site.
2. Visit the FlowJo registration site and register your computer.
3. An email will be sent to you with a serial number and instructions for downloading FlowJo.

Questions?

Call Monica DeLay at 513-636-3575.