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Anil Mishra, PhDAssociate Professor Department of Pediatrics; Division of Allergy and Immunology
The goal of Dr. Mishra’s research investigates novel pharmaceutical targets for the treatment of patients with eosinophilic diseases including eosinophilic gastrointestinal disorders and food allergies. The laboratory has identified and biologically characterized several critical pathways that regulate allergic responses. Dr. Mishra’ laboratory is presently focused on the role of invariant natural killer (iNKT) cells and its growth, survival and activating cytokines IL-15 and IL-18 and chemokines CXCL16. Most recently, he demonstrated that esophageal biopsies of EE patients have increased expression of IL-15 mRNA. Induced IL-15 expression significantly correlates with esophageal eosinophilia in humans and, following treatment IL-15 levels were reduced in improved EE patient. Furthermore he demonstrated that, IL-15R gene-deficient mice are protected from the induction of EE. His recent investigations suggest that most T cells accumulated in the esophagus of EE patients are iNKT cells, which indicates their role in EE pathogenesis and subject is under investigation.
Dr. Mishra has used the Bioinformatics, Microarray, and Integrative Morphology Cores in collaboration with Drs. Aronow, Hogan, Rothenberg, and Witte examining the RNA transcripts that are responsible for eosinophilic esophagitis. Anticipated use of Cores: Integrative Morphology, Gene and Protein Expression, and Bioinformatics Cores.
click image to enlarge
Immunoreactivity of IL-15 was tested on esophageal biopsy specimens from non-EoE individuals and patients with EoE by performing immunohistochemistry. No IL-15–positive cells were detected in non-EoE patient esophageal biopsy specimens (arrows, original magnification 10X (i), inset magnification 400X(ii). A number of infiltrating cells were detected positive for IL-15 in the esophageal biopsy specimens from patients with EoE (original magnification 10X (iii) and inset magnification 400X (iv). Figure from Gastroenterology 2010;139:182-193.
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