Pluripotent Stem Cell and Organoid Core
The objective of this core is to assist Digestive Health Center (DHC) investigators to use state-of-the-art technology of human pluripotent stem cells and organoids to advance translational research in digestive diseases. The services are provided by the staff of the CCHMC Pluripotent Stem Cell Facility (PSCF) under the leadership of Dr. Chris Mayhew and Dr. James Wells.
Services:
- Generation of induced pluripotent stem cells (iPSCs) from DHC-relevant patient cells
- Generates 6 frozen vials from up to 6 discrete iPSC lines from a single donor of fibroblasts or PBMCs
- Directed differentiation of human pluripotent stem cells (hPSCs) to human intestinal organoids (HIOs
- Generates 12 wells with each well containing at least 2 HIO
Training:
- Basic Training course to learn how to culture human pluripotent stem cells
- Advanced Training in Generation of iPSC from DHC-relevant patient Cells
- Advanced Training in Directed Differentiation of hPSCs to HIOs
Pricing:
- Basic Training course to learn how to culture human pluripotent stem cells is provided by the DHC (cost savings of $850).
- The DHC will subsidize the cost of the Pluripotent Stem Cell and Organoid Core services by providing up to $2,000 towards the total charge per PI per year.
For more information and a complete list of services offered by the PSCF core visit the PSCF website or email the core at PSCF@cchmc.org.
