• Pluripotent Stem Cell and Organoid Core

    The objective of this core is to assist Digestive Health Center (DHC) investigators to use state-of-the-art technology of human pluripotent stem cells and organoids to advance translational research in digestive diseases. The services are provided by the staff of the CCHMC Pluripotent Stem Cell Facility (PSCF) under the leadership of Dr. Chris Mayhew and Dr. James Wells.

    Services

    • Generation of human induced pluripotent stem cells (iPSCs) from DHC-relevant patient cells

      • iPSC derivation from fibroblast (3-6 colonies/sample)             $2,150
      • iPSC derivation from Peripheral Blood Mononuclear Cells (3-6 colonies/sample)               $2,650
    • Directed differentiation of human pluripotent stem cells (hPSCs) to human intestinal organoids (HIOs)

      • Generates 12 wells (minimum 2 HIOs per well)                      $395

    Training

    • Basic Training Course in the techniques for
      culturing human pluripotent stem cells                                          $850

    • Advanced Training

      • iPSC derivation from fibroblast                                            $2,850
      • iPSC derivation from PBMCs                                              $3,350
      • Differentiation of hPSCs to Human Intestinal Organoids        $625

    Pricing for DHC Members

    • Basic Training course to learn how to culture human pluripotent stem cells is provided by the DHC.
    • The DHC will subsidize the cost of the services at the Pluripotent Stem Cell and Organoid Core by providing 50% of the total charge up to $2,000 per PI per year. This subsidy is subject to change based upon the availability of funds.


    For more information and a complete list of services offered by the PSCF core, visit the PSCF website or email the core at PSCF@cchmc.org

  • DHC logo

  • Contact Us

    Director

    James Wells PhD
    Location S3.621
    Phone: 513-636-8767
    Email: james.wells@cchmc.org

    Co-Director

    Christopher Mayhew, PhD
    Location R3025
    Phone: 513-636-9254
    Email: christopher.mayhew@cchmc.org

    Location of the Core:

    R3023

  • Have you used the DHC Pluripotent Stem Cell and Organoid Core?

    Remember to acknowledge the use of the core facility in all publications by stating:

    "This project was supported in part by NIH P30 DK078392 (Pluripotent Stem Cell and Organoid Core of the Digestive Disease Research Core Center in Cincinnati)."