Integrative Morphology Core
Director: David Witte, MD
Research Pathology Service
Personnel will provide technical support for routine morphology based techniques including tissue processing, routine histology, in situ hybridization, immunohistochemistry, routine and special histochemical staining, photography, and electron microscopy support.
The Integrative Morphology Core will also provide experienced microscopic analyses, extensive pathologic evaluation and consultation to the investigators regarding the interpretation of morphology data as well as guidance regarding experimental design.
Strategy and Experimental Design
Remember that Consulting is Free.
Discussing what you want to do with the Integrative Core Staff for 30 minutes before you start your experiment might save lots of time in the end.
Contact Pam Groen to set up an appointment
Phone 513-636-8445
Email pam.groen@cchmc.org
Routine Histology
Contact: Meredith Taylor
Phone: 513-636-8159
Email: meredith.taylor@cchmc.org
Scanning Electron Microscopy
Contact: Pam Groen
Phone: 513-636-8445
Email: pam.groen@cchmc.org
Transmission Electron Microscopy
Contact: Jan Kripp
Phone: 513-803-0739
Email: jannene.kripp@cchmc.org
Pathology Support
Contact: Keith Stringer, MD
Phone: 513-636-9876
Email: keith.stringer@cchmc.org
Photography Support
Contact: Chris Woods
Phone: 513-636-1892
Email: chris.woods@cchmc.org
In order to receive the discounts from research pathology services (all services except Live Microscopy and Laser Capture Microdissection) you must use the Biological Sample Tracking System (BSTS). The BSTS allows the investigator, individual cores, and DHC administrators to keep track of the experimental projects and samples.
BSTS Web Address Inside CCHMC: http://info.cchmc.org/bsts
BSTS Web Address Outside of CCHMC: https://bsts.cchmc.org/
* You must acquire a username and password to get through the CCHMC firewall. Please contact the Pathology Lab at 636-8159 for more information.
BSTS Training
Contact: Lisa McMillin
Phone: 513-636-8159
Email: lisa.mcmillin@cchmc.org
Live Microscopy Service
The Live Microscopy facility has 2 advanced Zeiss confocal microscopes for use, one of which has the additional equipment for two-photon microscopy. Two-photon excitation produces images with the same resolution as confocal imaging, but is applicable for deep tissue imaging in living specimens and/or local deposition of energy in three dimensions for other purposes (uncaging of fluorophores, targeted cell ablation, etc.) For more information visit the Live Microscopy web page.
Director of Live Microscopy Service
Marshall (Chip) Montrose, PhD
Phone: 513-558-5636
Email: mhm@uc.edu
Live Microscopy Service Facility Manager
Chet Closson
Phone: 513-558-3791
Email: chet.closson@uc.edu
Laser Capture Microdissection Service
There are currently 2 Laser Capture Microdissection instruments.
- Acturus Veritas microdissection system has both a UV cutting laser and IR capture laser which allows much more experimental versatility. Extensive computer controls increase the automation of sample handling and capture that is available in the Veritas system which allows users to work more effectively and productively. This system also has improved fluorescence capability.
- PixCell II LCM System from Acturus Engineering has fluorescence capability. For more information visit the Laser Capture Microdissection web page.
Laser Capture Microdissection Service Facility Manager
Xioanan Han, PhD
Phone: 513-636-7592
Pager: 513-736-3391
Email: xioanan.han@cchmc.org
| Service | Charges for DHC Member | Charges for non DHC Member |
|---|
| Tissue processing and embedding |
No charge |
$15 minimum (first 15 blocks), $1/ additional block |
| Paraffin or cryostat sectioning |
$1.50/slide |
$2.00/slide |
| H&E Staining |
$2.50/slide |
$4.50/slide |
| Special staining |
$3.50/slide |
$6.50/slide |
| Immunohistochemistry |
$10.00/slide |
$18/slide |
| In situ hybridization |
$1000/50 slides |
$1500/50 slides |
| Electron microscopy tissue processing |
$20/10 blocks |
$40/10 blocks |
| Electron microscopy sectioning |
$20/hour |
$30/hour |
| Electron microscope usage |
No charge |
$50/hour |
| Pathologist slide interpretation |
No charge |
$100/hour |
| Photography support |
No charge |
$20/hour plus $3/print |
Live Microscopy Service
Zeiss Two-Photon Microscope
Zeiss META Confocal Microscope |
2 free user trainings per PI per year |
User training costs $250/person |
| |
First time PIs receive 20 hours free. Then $20/hr weekdays 8-5, $10/hr night/weekends
(this discount is applied to first 100 hrs per quarter) |
$25/hr weekdays 8-5, $15/hr night/weekends |
| Laser Capture Microdissection |
No charge for user training |
User training costs approximately $48-96 |
| |
No charge for first 10 hours then $16/hr |
$24/hr |
Remember to identify the DHC (P30 DK078392) in all publications resulting from core supported work.
Last updated: May 21, 2008
