Integrative Morphology Core
Director: David Witte, MD
Research Pathology Service | Live Microscopy Service | Laser Capture Microdissection Service
Research Pathology Service
Personnel will provide technical support for routine morphology based techniques including tissue processing, routine histology, in situ hybridization, immunohistochemistry, routine and special histochemical staining, photography, and electron microscopy support.
The Integrative Morphology Core will also provide experienced microscopic analyses, extensive pathologic evaluation and consultation to the investigators regarding the interpretation of morphology data as well as guidance regarding experimental design.
Strategy and Experimental Design
Remember that Consulting is Free.
Discussing what you want to do with the Integrative Core Staff for 30 minutes before you start your experiment might save lots of time in the end.
Contact Pam Groen to set up an appointment
Phone 513-636-8445
Email pam.groen@cchmc.org
Routine Histology
Contact: Meredith Taylor
Phone: 513-636-8159
Email: meredith.taylor@cchmc.org
Scanning Electron Microscopy
Contact: Pam Groen
Phone: 513-636-8445
Email: pam.groen@cchmc.org
Transmission Electron Microscopy
Contact: Jan Kripp
Phone: 513-803-0739
Email: jannene.kripp@cchmc.org
Pathology Support
Contact: Keith Stringer, MD
Phone: 513-636-9876
Email: keith.stringer@cchmc.org
Photography Support
Contact: Chris Woods
Phone: 513-636-1892
Email: chris.woods@cchmc.org
| Service |
Charges for DHC Member per fiscal year of DHC grant |
Charges for non DHC Member |
| Tissue processing and embedding |
No charge for the first 1,500 blocks |
$15 minimum (first 15 blocks), $1/ additional block |
| Paraffin or cryostat sectioning |
$1.50/slide for the first 4,500 slides |
$2.00/slide |
| H&E Staining |
$2.50/slide for the first 1,000 slides |
$4.50/slide |
| Special staining |
$3.50/slide for the first 500 slides |
$6.50/slide |
| Immunohistochemistry |
$10.00/slide for the first 500 slides |
$18/slide |
| In situ hybridization |
$20/slide for the first 100 slides |
$30/slide |
| Electron microscopy tissue processing |
$20/10 blocks |
$40/10 blocks |
| Electron microscopy sectioning |
$20/hour |
$30/hour |
| Electron microscope usage |
No charge |
$50/hour |
| Pathologist slide interpretation |
No charge |
$100/hour |
| Photography support |
No charge |
$20/hour plus $3/print |
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Live Microscopy Service
The Live Microscopy facility has 2 advanced Zeiss confocal microscopes for use, one of which has the additional equipment for two-photon microscopy. Two-photon excitation produces images with the same resolution as confocal imaging, but is applicable for deep tissue imaging in living specimens and/or local deposition of energy in three dimensions for other purposes (uncaging of fluorophores, targeted cell ablation, etc.) For more information visit the Live Microscopy web page.
Director of Live Microscopy Service
Marshall (Chip) Montrose, PhD
Phone: 513-558-5636
Email: mhm@uc.edu
Live Microscopy Service Facility Manager
Chet Closson
Phone: 513-558-3791
Email: chet.closson@uc.edu
Location of Confocal Microscopes
University of Cincinnati MSB Room 4105
| Service |
Charges for DHC Member per fiscal year of DHC grant |
Charges for non DHC Member |
Training
|
2 free user trainings per PI per year
|
User training costs $250/person
|
Microscope Time
Zeiss Two-Photon Microscope
Zeiss META Confocal Microscope |
First time PIs receive 20 hours free.
Then $20/hr weekdays 8-5, $10/hr night/weekends
(this discount is applied to first 100 hrs per quarter)
|
$30/hr weekdays 8-5,
$20/hr night/weekends
|
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Laser Capture Microdissection Service
There are currently 2 Laser Capture Microdissection instruments.
- Acturus Veritas microdissection system has both a UV cutting laser and IR capture laser which allows much more experimental versatility. Extensive computer controls increase the automation of sample handling and capture that is available in the Veritas system which allows users to work more effectively and productively. This system also has improved fluorescence capability.
- PixCell II LCM System from Acturus Engineering has fluorescence capability. For more information visit the Laser Capture Microdissection web page.
Laser Capture Microdissection Service Facility Manager
Xioanan Han, PhD
Phone: 513-636-7592
Pager: 513-736-3391
Email: xioanan.han@cchmc.org
Location of Microdissection System
Cincinnati Children's Hospital Medical Center Building R Room 6529
| Service |
Charges for DHC Member per fiscal year of DHC grant |
Charges for non DHC Member |
| Training |
No charge for user training
|
User training costs approximately $48-96
|
Instrument Time
Acturus Veritas System
PixCell II LCM System |
No charge for first 10 hours then $16/hr
|
$24/hr
|
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Remember to acknowledge the use of the Integrative Morphology core in all publications by stating:
"This project was supported in part by PHS Grant P30 DK078392."
Last updated: September 10, 2009
