Christopher Mayhew, PhD
Director
christopher.mayhew@cchmc.org

The Cincinnati Children's Pluripotent Stem Cell Facility facilitates and supports studies using human pluripotent stem cells. Services include routine access to quality-controlled hESC and iPSC lines, generation of patient-specific iPSCs, CRISPR-Cas9 mediated genome editing of iPSCs, and hands-on training modules in the maintenance of high-quality hESC/iPSCs and differentiation into GI organoids.

Katherine Lane
Lab Support Tech I
katherine.lane@cchmc.org

Kate is currently studying chemistry at the University of Cincinnati. She has been with the PSCF since 2018.

Kyle MacKenzie, BS, AS
Research Assistant III
kyle.mackenzie@cchmc.org

Kyle obtained his associates degree in bioscience technology from Cincinnati State in 2015 and his bachelor degree in biology at University of Cincinnati in 2018. He started his career at Cincinnati Children's Hospital Medical Center in 2014 as a lab aide and joined the PSCF in 2019. He works on iPSC generation, banking and QC for the Pluripotent Stem Cell Facility and CuSTOM.

Isheunouya (Ishe) Mazani, BS
Research Assistant III
isheunouya.mazani@cchmc.org

Ishe graduated with his bachelor of science in biology in 2017. He joined the PSCF in the fall of 2021 where he is member of iPSC gene editing team. Prior to working at Cincinnati Children's he worked in a clinical lab at Miami Valley Hospital in Dayton, OH.

Amy Pitstick, MS
Research Assistant IV, Lab Manager
amy.pitstick@cchmc.org

Amy earned her master’s in biology from Wright State University. Amy joined the Pluripotent Stem Cell Facility in 2012 as a research assistant, focusing predominately on the generation of iPSCs for investigators, as well as training other researchers interested in utilizing the iPSC culture system. Recently, her focus has shifted towards the implementation and optimization of protocols for both iPSC generation as well as iPSC-derived organoids.

Joseph Shiley
Research Assistant III
joseph.shiley@cchmc.org

Megan Slack
Research Assistant III
megan.slack@cchmc.org