Single Cell Genomics Facility

Techniques and Services

The SCGF utilizes 10x Genomics technology to perform single-cell RNA sequencing (mRNA-Seq), single-cell chromatin accessibility sequencing (ATAC-Seq), and single-cell Multiome sequencing (combining ATAC-Seq and RNA expression analysis).

The SCGF currently provides 10x Genomics whole-transcriptome single-cell resolution Spatial Transcriptomics services from both FFPE and frozen tissue samples. They are actively expanding their spatial platform to include panel-based in situ subcellular profiling for whole transcriptome validation.

For projects with restricted starting RNA quantities, the SCGF offers RNA-Seq assays from Tecan Genomics, Takara, and Illumina.

The SCGF isolates and checks the RNA quality of tissue scrolls from OCT or FFPE tissue blocks.

The SCGF offers quality assessments for isolated and purified RNA and DNA.