A photo of Scott Witting.

Director, Translational Trials Support & Development Laboratory

Assistant Professor, UC Department of Pediatrics

513-803-1066

513-636-1446

Biography & Affiliation

Biography

My areas of interest include cell and gene therapy manufacturing techniques and quality control of cell and gene therapy products. With my work, I hope to generate assays to characterize the safety and efficacy of cell and gene therapy products.

I became interested in this field during my postdoctoral fellowship studies which focused on using adenoviral vectors expressing short hairpin RNA (shRNA) to silence gene expression in mouse liver cells. Although the goal of this work was to develop a tool to study liver gene function, it sparked my interest in gene therapy. Subsequently, I created a novel lentivirus vector pseudotype based on the Nipah virus. I also developed a new method for large-scale clinical-grade lentivirus production using flow electroporation to overcome manufacturing roadblocks.

Upon encountering a challenging funding climate in the early to mid-2010s, I accepted an opportunity to become the associate director of the Indiana University Vector Production Facility. Due to my previous work experience and successful collaborations, I successfully transitioned from an academic investigator to managing a gene therapy vector manufacturing and testing facility.

Now, at Cincinnati Children’s Hospital Medical Center, I direct a translational testing laboratory specializing in developing and performing assays for cell and gene therapy clinical trial monitoring.

Our laboratory meets all Clinical Laboratory Improvement Amendments (CLIA) standards and is accredited by the College of American Pathologists (CAP). We also meet all Good Manufacturing Practice (GMP) requirements for early phase clinical trial work.

I have served on two National Institutes of Health steering committees, including one for the National Heart, Lung, and Blood Institute, that provided support to academic investigators with early phase cell and gene therapy clinical trials. I am a member of the Gene Therapy Resource Program, and I serve as the translational technology committee chair for the Growing Gene and Cell Therapy Cooperative.

I look forward to further contributions to the growth of cell and gene therapy at Cincinnati Children’s. These therapies are often the best, if not the only, treatment available for an ever-increasing list of cancers and monogenetic diseases.

Clinical Interests

Clinical assay development; gene and cell therapy assay development and analysis

Academic Affiliation

Assistant Professor, UC Department of Pediatrics

Research Divisions

Experimental Hematology and Cancer Biology

Education

BS: Biology, University of Detroit Mercy, Detroit, MI, 1998.

PhD: Pathology and Molecular Medicine, University of Cincinnati, Cincinnati, OH, 2004.

Post-doctoral: Type 2 Diabetes and Gene Therapy, Indiana University School of Medicine, Indianapolis, IN, 2007.

Publications

A scalable lentiviral vector production and purification method using mustang Q chromatography and tangential flow filtration. Tinch, S; Szczur, K; Swaney, W; Reeves, L; Witting, SR. Methods in molecular biology (Clifton, N.J.). 2019; 1937:135-153.

Differences in vector-genome processing and illegitimate integration of non-integrating lentiviral vectors. Shaw, AM; Joseph, GL; Jasti, AC; Sastry-Dent, L; Witting, S; Cornetta, K. Gene Therapy (Basingstoke). 2017; 24:12-20.