Whitsett Lab

Publications

Thebaud, B; Goss, KN; Laughon, M; Whitsett, JA; Abman, SH; Steinhorn, RH; Aschner, JL; Davis, PG; McGrath-Morrow, SA; Soll, RF; Jobe, AH. Bronchopulmonary dysplasia. Nature Reviews. Disease Primers. 2019; 5:78.

Guo, M; Du, Y; Gokey, JJ; Ray, S; Bell, SM; Adam, M; Sudha, P; Perl, AK; Deshmukh, H; Potter, SS; Whitsett, JA; Xu, Y. Single cell RNA analysis identifies cellular heterogeneity and adaptive responses of the lung at birth. Nature Communications. 2019; 10:37.

Ren, X; Ustiyan, V; Guo, M; Wang, G; Bolte, C; Zhang, Y; Xu, Y; Whitsett, JA; Kalin, TV; Kalinichenko, VV. Postnatal Alveologenesis Depends on FOXF1 Signaling in c-KIT+ Endothelial Progenitor Cells. American Journal of Respiratory and Critical Care Medicine. 2019; 200:1164-1176.

Pradhan, A; Dunn, A; Ustiyan, V; Bolte, C; Wang, G; Whitsett, JA; Zhang, Y; Porollo, A; Hu, Y; Xiao, R; Szafranski, P; Shi, D; Stankiewicz, P; Kalin, TV; Kalinichenko, VV. The S52F FOXF1 Mutation Inhibits STAT3 Signaling and Causes Alveolar Capillary Dysplasia. American Journal of Respiratory and Critical Care Medicine. 2019; 200:1045-1056.

Du, Y; Clair, GC; Al Alam, D; Danopoulos, S; Schnell, D; Kitzmiller, JA; Misra, RS; Bhattacharya, S; Warburton, D; Mariani, TJ; Pryhuber, GS; Whitsett, JA; Ansong, C; Xu, Y. Integration of transcriptomic and proteomic data identifies biological functions in cell populations from human infant lung. American Journal of Physiology: Lung Cellular and Molecular Physiology. 2019; 317:L347-L360.

Whitsett, JA; Kalin, TV; Xu, Y; Kalinichenko, VV. Building and Regenerating the Lung Cell by Cell. Physiological Reviews. 2019; 99:513-554.

Gokey, JJ; Snowball, J; Sridharan, A; Speth, JP; Black, KE; Hariri, LP; Perl, AT; Xu, Y; Whitsett, JA. MEG3 is increased in idiopathic pulmonary fibrosis and regulates epithelial cell differentiation. JCI insight. 2018; 3:e122490.

Tang, X; Snowball, JM; Xu, Y; Na, C; Weaver, TE; Clair, G; Kyle, JE; Zink, EM; Ansong, C; Wei, W; Huang, M; Lin, X; Whitsett, JA. EMC3 coordinates surfactant protein and lipid homeostasis required for respiration. The Journal of Clinical Investigation. 2017; 127:4314-4325.

Gray, J; Oehrle, K; Worthen, G; Alenghat, T; Whitsett, J; Deshmukh, H. Intestinal commensal bacteria mediate lung mucosal immunity and promote resistance of newborn mice to infection. Science Translational Medicine. 2017; 9:eaaf9412.

Whitsett, JA; Morrisey, EE. INFLAMMATION. Modulating pulmonary inflammation. Science. 2016; 351:662-663.

Tang, X; Wei, W; Sun, Y; Weaver, TE; Nakayasu, ES; Clair, G; Snowball, JM; Na, CL; Apsley, KS; Martin, EP; Molkentin, JD; Gower, WA; Lin, X; Whitsett, JA. EMC3 regulates trafficking and pulmonary toxicity of the SFTPCI73T mutation associated with interstitial lung disease. The Journal of Clinical Investigation. 2024; 134:e173861.

Wang, G; Wen, B; Guo, M; Li, E; Zhang, Y; Whitsett, JA; Kalin, TV; Kalinichenko, VV. Identification of endothelial and mesenchymal FOXF1 enhancers involved in alveolar capillary dysplasia. Nature Communications. 2024; 15:5233.

He, H; Bell, SM; Davis, AK; Zhao, S; Sridharan, A; Na, CL; Guo, M; Xu, Y; Snowball, J; Swarr, DT; Zacharias, WJ; Whitsett, JA. PRDM3/16 regulate chromatin accessibility required for NKX2-1 mediated alveolar epithelial differentiation and function. Nature Communications. 2024; 15:8112.

Whitsett, JA; Sever, Z. Respiratory Epithelial Cell Gene Transcription. Lung Growth and Development. : Taylor & Francis; Taylor & Francis; 2024.

Bhattacharya, S; Myers, JA; Baker, C; Guo, M; Danopoulos, S; Myers, JR; Bandyopadhyay, G; Romas, ST; Huyck, HL; Misra, RS; Whitsett, JA; Xu, Y; Pryhuber, GS; Mariani, TJ. Single-Cell Transcriptomic Profiling Identifies Molecular Phenotypes of Newborn Human Lung Cells. Genes. 2024; 15:298.

Bzdęga, K; Biela, M; Deutsch, GH; Kitzmiller, JA; Rydzanicz, M; Płoski, R; Whitsett, JA; Śmigiel, R; Karolak, JA. A novel non-recurrent CNV deletion involving TBX4 and leaving TBX2 intact causes congenital alveolar dysplasia. Clinical Genetics: an international journal of genetics and molecular medicine. 2024; 105:190-195.

Gaddis, N; Fortriede, J; Guo, M; Bardes, EE; Kouril, M; Tabar, S; Burns, K; Ardini-Poleske, ME; Loos, S; Schnell, D; Xu, Y; Tickle, T; Aronow, BJ; Salomonis, N. LungMAP Portal Ecosystem: Systems-level Exploration of the Lung. American Journal of Respiratory Cell and Molecular Biology. 2024; 70:129-139.

Wambach, JA; Wegner, DJ; Kitzmiller, J; White, FV; Heins, HB; Yang, P; Paul, AJ; Granadillo, JL; Eghtesady, P; Kuklinski, C; Schroeder, MC; Neidich, JA; Whitsett, JA; Cole, FS. Homozygous, Intragenic Tandem Duplication of SFTPB Causes Neonatal Respiratory Failure. American Journal of Respiratory Cell and Molecular Biology. 2024; 70:78-80.

Guo, M; Morley, MP; Jiang, C; Wu, Y; Li, G; Du, Y; Zhao, S; Wagner, A; Cakar, AC; Kouril, M; Morrisey, EE; Whitsett, JA; Lin, S; Xu, Y. Guided construction of single cell reference for human and mouse lung. Nature Communications. 2023; 14:4566.

Kim, H; Yi, S; Wikenheiser-Brokamp, K; Whitsett, J; Naren, A; Mun, K. 157 Patient-derived 3-D lung airway model to study mucociliary clearance. Journal of Cystic Fibrosis. 2023; 22:s83.

Huang, Y; Penrod, S; Whitsett, J; Naren, A. 331 Endoplasmic reticulum membrane protein complex is required for CFTR biogenesis and activation in vivo. Journal of Cystic Fibrosis. 2023; 22:s165.

Guo, M; Wikenheiser-Brokamp, KA; Kitzmiller, JA; Jiang, C; Wang, G; Wang, A; Preissl, S; Hou, X; Buchanan, J; Karolak, JA; Stankiewicz, P; Kalinichenko, VV; Wambach, JA; Whitsett, JA. Single Cell Multiomics Identifies Cells and Genetic Networks Underlying Alveolar Capillary Dysplasia. American Journal of Respiratory and Critical Care Medicine. 2023; 208:709-725.

Sikkema, L; Ramírez-Suástegui, C; Strobl, DC; Gillett, TE; Zappia, L; Madissoon, E; Markov, NS; Zaragosi, LE; Ji, Y; Ansari, M; Misharin, AV; Nawijn, MC; Luecken, MD; Theis, FJ. An integrated cell atlas of the lung in health and disease. Nature Medicine. 2023; 29:1563-1577.

Olatoke, T; Wagner, A; Astrinidis, A; Zhang, EY; Guo, M; Zhang, AG; Mattam, U; Kopras, EJ; Gupta, N; Smith, EP; Whitsett, JA; McCormack, FX; Xu, Y; Yu, JJ. Single-cell multiomic analysis identifies a HOX-PBX gene network regulating the survival of lymphangioleiomyomatosis cells. Science Advances. 2023; 9:eadf8549.

Huang, Y; Penrod, S; Whitsett, J; Naren, AP. Su1232 EMC REGULATES INTESTINAL EPITHELIAL STEM CELL HOMEOSTASIS PARTIALLY THROUGH CALCIUM MOBILIZATION. Gastroenterology. 2023; 164:s-560.

Karolak, JA; Welch, CL; Mosimann, C; Bzdęga, K; West, JD; Montani, D; Eyries, M; Mullen, MP; Abman, SH; Prapa, M; Galambos, C; Stankiewicz, P; Chung, WK; Austin, ED. Molecular Function and Contribution of TBX4 in Development and Disease. American Journal of Respiratory and Critical Care Medicine. 2023; 207:855-864.

Tang, X; Wei, W; Snowball, JM; Nakayasu, ES; Bell, SM; Ansong, C; Lin, X; Whitsett, JA. EMC3 regulates mesenchymal cell survival via control of the mitotic spindle assembly. iScience. 2023; 26:105667.

Huang, Y; Whitsett, J; Naren, A. 667 Endoplasmic reticulum membrane protein complex is required for cystic fibrosis transmembrane conductance regulator biogenesis and activation in vivo. Journal of Cystic Fibrosis. 2022; 21:s364-s365.

Toth, A; Steinmeyer, S; Kannan, P; Gray, J; Jackson, CM; Mukherjee, S; Demmert, M; Sheak, JR; Benson, D; Kitzmiller, J; Chougnet, CA; Jobe, AH; Deshmukh, H; Zacharias, WJ. Inflammatory blockade prevents injury to the developing pulmonary gas exchange surface in preterm primates. Science Translational Medicine. 2022; 14:eabl8574.

Clair, G; Bramer, LM; Misra, R; McGraw, MD; Bhattacharya, S; Kitzmiller, JA; Feng, S; Danna, VG; Bandyopadhyay, G; Bhotika, H; Whitsett, JA; Pryhuber, GS; Adkins, JN; Ansong, C. Proteomic Analysis of Human Lung Development. American Journal of Respiratory and Critical Care Medicine. 2022; 205:208-218.

Sun, X; Perl, AK; Li, R; Bell, SM; Sajti, E; Kalinichenko, VV; Kalin, TV; Misra, RS; Deshmukh, H; Clair, G; Xu, Y; Pryhuber, G; Whitsett, J; Morrisey, EE. A census of the lung: CellCards from LungMAP. Developmental Cell. 2022; 57:112-145.e2.

Kingma, PS; Guiot, N; Whitsett, JA. Pulmonary Collectins☆. Encyclopedia of Respiratory Medicine. : Elsevier; Elsevier; 2022.

Boat, TF; Whitsett, JA. How Can the Pediatric Community Enhance Funding for Child Health Research?. JAMA pediatrics. 2021; 175:1212-1214.

Bui, LT; Winters, NI; Chung, MI; Joseph, C; Gutierrez, AJ; Habermann, AC; Adams, TS; Schupp, JC; Poli, S; Peter, LM; Biancalani, T; Desai, T; Sun, X; Zaragosi, LE. Chronic lung diseases are associated with gene expression programs favoring SARS-CoV-2 entry and severity. Nature Communications. 2021; 12:4314.

Kolesnichenko, OA; Whitsett, JA; Kalin, TV; Kalinichenko, VV. Therapeutic Potential of Endothelial Progenitor Cells in Pulmonary Diseases. American Journal of Respiratory Cell and Molecular Biology. 2021; 65:473-488.

Sun, YL; Hurley, K; Villacorta-Martin, C; Huang, J; Hinds, A; Gopalan, K; Caballero, IS; Russo, SJ; Kitzmiller, JA; Whitsett, JA; Beers, MF; Kotton, DN. Heterogeneity in Human Induced Pluripotent Stem Cell-derived Alveolar Epithelial Type II Cells Revealed with ABCA3/SFTPC Reporters. American Journal of Respiratory Cell and Molecular Biology. 2021; 65:442-460.

Gokey, JJ; Snowball, J; Sridharan, A; Sudha, P; Kitzmiller, JA; Xu, Y; Whitsett, JA. YAP regulates alveolar epithelial cell differentiation and AGER via NFIB/KLF5/NKX2-1. iScience. 2021; 24:102967.

Wang, G; Wen, B; Ren, X; Li, E; Zhang, Y; Guo, M; Xu, Y; Whitsett, JA; Kalin, TV; Kalinichenko, VV. Generation of Pulmonary Endothelial Progenitor Cells for Cell-based Therapy Using Interspecies Mouse-Rat Chimeras. American Journal of Respiratory and Critical Care Medicine. 2021; 204:326-338.

Li, E; Ustiyan, V; Wen, B; Kalin, GT; Whitsett, JA; Kalin, TV; Kalinichenko, VV. Blastocyst complementation reveals that NKX2-1 establishes the proximal-peripheral boundary of the airway epithelium. Developmental Dynamics. 2021; 250:1001-1020.

Rindler, TN; Brown, KM; Stockman, CA; van Lieshout, LP; Martin, EP; Weaver, TE; Zacharias, WJ; Wootton, SK; Whitsett, JA; Bridges, JP. Efficient Transduction of Alveolar Type 2 Cells with Adeno-associated Virus for the Study of Lung Regeneration. American Journal of Respiratory Cell and Molecular Biology. 2021; 65:118-121.

Jiang, M; Fang, Y; Li, Y; Huang, H; Wei, Z; Gao, X; Sung, HK; Hu, J; Qiang, L; Ruan, J; Chen, Q; Jiang, D; Whitsett, JA; Ai, X; Que, J. VEGF receptor 2 (KDR) protects airways from mucus metaplasia through a Sox9-dependent pathway. Developmental Cell. 2021; 56:1646-1660.e5.

Whitsett, JA; Jobe, AH. Commentary on the Truncated Splice Variant of the GM-CSF Receptor Beta-Chain in Peripheral Blood Serves as Severity Biomarker of Respiratory Failure in Newborns. Neonatology: foetal and neonatal research. 2021; 118:194-197.

He, H; Snowball, J; Sun, F; Na, CL; Whitsett, JA. IGF1R controls mechanosignaling in myofibroblasts required for pulmonary alveologenesis. JCI insight. 2021; 6:e144863.

Muus, C; Luecken, MD; Eraslan, G; Sikkema, L; Waghray, A; Heimberg, G; Kobayashi, Y; Vaishnav, ED; Subramanian, A; Smillie, C; Smith, NP; Sountoulidis, A; Strunz, M; Sullivan, TB. Single-cell meta-analysis of SARS-CoV-2 entry genes across tissues and demographics. Nature Medicine. 2021; 27:546-559.

Wu, G; Lee, YY; Gulla, EM; Potter, A; Kitzmiller, J; Ruben, MD; Salomonis, N; Whitsett, JA; Francey, LJ; Hogenesch, JB; Smith, DF. Short-term exposure to intermittent hypoxia leads to changes in gene expression seen in chronic pulmonary disease. eLife. 2021; 10:e63003.

Wen, B; Li, E; Ustiyan, V; Wang, G; Guo, M; Na, CL; Kalin, GT; Galvan, V; Xu, Y; Weaver, TE; Kalin, TV; Whitsett, JA; Kalinichenko, VV. In Vivo Generation of Lung and Thyroid Tissues from Embryonic Stem Cells Using Blastocyst Complementation. American Journal of Respiratory and Critical Care Medicine. 2021; 203:471-483.

Du, Y; Ouyang, W; Kitzmiller, JA; Guo, M; Zhao, S; Whitsett, JA; Xu, Y. Lung Gene Expression Analysis Web Portal Version 3: Lung-at-a-Glance. American Journal of Respiratory Cell and Molecular Biology. 2021; 64:146-149.

Bernardes, JP; Mishra, N; Tran, F; Bahmer, T; Best, L; Blase, JI; Bordoni, D; Franzenburg, J; Geisen, U; Josephs-Spaulding, J; Whitsett, J; Zaragosi, LE; Angelov, A; Bals, R. Longitudinal Multi-omics Analyses Identify Responses of Megakaryocytes, Erythroid Cells, and Plasmablasts as Hallmarks of Severe COVID-19. Immunity. 2020; 53:1296-1314.e9.

Kang, MH; van Lieshout, LP; Xu, L; Domm, JM; Vadivel, A; Renesme, L; Muhlfeld, C; Hurskainen, M; Mizikov, I; Pei, Y; Whitsett, JA; Nogee, LM; Wootton, SK; Thebaud, B. A lung tropic AAV vector improves survival in a mouse model of surfactant B deficiency. Nature Communications. 2020; 11:3929.

Guo, M; Yu, JJ; Perl, AK; Wikenheiser-Brokamp, KA; Riccetti, M; Zhang, EY; Sudha, P; Adam, M; Potter, A; Kopras, EJ; Kwiatkowski, DJ; Whitsett, JA; McCormack, FX; Xu, Y. Single-Cell Transcriptomic Analysis Identifies a Unique Pulmonary Lymphangioleiomyomatosis Cell. American Journal of Respiratory and Critical Care Medicine. 2020; 202:1373-1387.

Wang, A; Chiou, J; Poirion, OB; Buchanan, J; Valdez, MJ; Verheyden, JM; Hou, X; Kudtarkar, P; Narendra, S; Newsome, JM; Xu, Y; Gaulton, KJ; Preissl, S; Sun, X. Single-cell multiomic profiling of human lungs reveals cell-type-specific and age-dynamic control of SARS-CoV2 host genes. eLife. 2020; 9:e62522.

Whitsett, JA; Glasser, SW. Targeting Gene Expression to the Lung. Gene Therapy for Diseases of the Lung. : Taylor & Francis; Taylor & Francis; 2020.

Cohen, SA; Kingma, PS; Whitsett, JA; Goldbart, R; Traitel, T; Kost, J. SP-D loaded PLGA nanoparticles as drug delivery system for prevention and treatment of premature infant's lung diseases. International Journal of Pharmaceutics. 2020; 585:119387.

Ziegler, CG K; Allon, SJ; Nyquist, SK; Mbano, IM; Miao, VN; Tzouanas, CN; Cao, Y; Yousif, AS; Bals, J; Hauser, BM; Rojas, M; Rosen, O; Saeb-Parsy, K; Samakovlis, C. SARS-CoV-2 Receptor ACE2 Is an Interferon-Stimulated Gene in Human Airway Epithelial Cells and Is Detected in Specific Cell Subsets across Tissues. Cell. 2020; 181:1016-1035.e19.

Sungnak, W; Huang, N; Bécavin, C; Berg, M; Queen, R; Litvinukova, M; Talavera-López, C; Maatz, H; Reichart, D; Sampaziotis, F; Zhang, H; Zhang, K; Rojas, M; Figueiredo, F. SARS-CoV-2 entry factors are highly expressed in nasal epithelial cells together with innate immune genes. Nature Medicine. 2020; 26:681-687.

Basil, MC; Katzen, J; Engler, AE; Guo, M; Herriges, MJ; Kathiriya, JJ; Windmueller, R; Ysasi, AB; Zacharias, WJ; Chapman, HA; Snoeck, H; Vunjak-Novakovic, G; Whitsett, JA; Morrisey, EE. The Cellular and Physiological Basis for Lung Repair and Regeneration: Past, Present, and Future. Cell Stem Cell. 2020; 26:482-502.