Bio-Imaging and Analysis Facility

Techniques and Services

Advanced Image Analysis

Proper analysis of datasets is critical to produce publications and secure funding. We offer training and assistance with data collected in our facility. We have nine image analysis workstations; all have NIS-Elements and five are designated for Bitplane Imaris. Some have advanced features such as trainable artificial intelligence, denoising, object tracking, deconvolution and batch processing.

Sample Preparation

We can assist with a variety of sample preparation techniques to increase resolution, including tissue clearing for large samples and expansion for sub-optical resolution.

Tissue clearing methods remove lipids to increase light penetration and increase resolution with larger samples. We provide training on a variety of passive and active tissue clearing techniques, including Spalteholz-like methods (BABB, DISCO, PEGASOS), aqueous methods (PACT, CLARITY, Ce3D), and newly developed protocols such as EZ Clear. Additionally, we maintain a Logos Biosystems X-Clarity machine if needed.

Expansion microscopy isotropically expands samples embedded in a hydrogel 4-10xs. This method is best utilized for biological phenomenon below the diffraction limit of light.