The Molecular Pathology (PCR) Laboratory, part of the Division of Pathology and Laboratory Medicine at Cincinnati Children’s, has the capability and expertise to perform an impressive range of infectious disease testing.  Additionally, our tests can be ordered on a wide range of specimen types.  As one of the first laboratories in the country to offer viral load monitoring for transplant patients, we bring a wealth of experience and skill to each sample we test.  See our About Us page for more information. 

Tests Available

The following tests are available:

  • Adenovirus (qualitative and quantitative)
  • Astrovirus
  • BK virus (qualitative and quantitative) 
  • Bordetella pertussis / parapertussis
  • Cryptosporidium spp.
  • Cytomegalovirus (CMV) (qualitative and quantitative) 
  • Epstein-Barr virus (EBV) (qualitative and quantitative) 
  • Enterovirus
  • Human Herpesvirus 6 (HHV-6) (qualitative and quantitative)
  • Herpes Simplex Virus (HSV) types 1 and 2 (qualitative and quantitative)
  • Norovirus 
  • Human Parechovirus (HPeV)
  • Parvovirus B19 (qualitative and quantitative)
  • Toxoplasma gondii 
  • Varicella-Zoster Virus (VZV)
  • Atypical pneumonia panel  
    • Chlamydia pneumoniae
    • Legionella pneumophila
    • Mycoplasma pneumoniae
  • Respiratory virus panel
    • Influenza virus A / B 
    • Parainfluenza virus 1, 2, 3, 4
    • Respiratory Syncytial Virus (RSV)
    • Human metapneumovirus
    • Human rhinovirus
    • Human coronavirus
  • BioFire FilmArray Meningitis/Encephalitis (M/E) Panel (includes Escherichia coli K1, Haemophilus influenza, Listeria monocytogenes, Neisseria meningitidis, Streptococcus agalactiae, Streptococcus pneumoniae, Cytomegalovirus, Herpes Simplex Virus 1, Herpes Simplex Virus 2, Human Herpesvirus 6, Enterovirus, Human Parechovirus, and Varicella-Zoster Virus, Cryptococcus gattii/neoformans)
  • BioFire FilmArray Syndromic Respiratory Panel (RP2) (includes Adenovirus, Coronavirus [HKU-1, NL63, 229E, OC43], Metapneumovirus, Rhinovirus/Enterovirus, Influenza A [H1, H1-2009, H3], Influenza B, Parainfluenza viruses 1, 2, 3, and 4, Respiratory Syncytial Virus, Bordetella pertussis, Bordetella parapertussis, Chlamydia pneumoniae, Mycoplasma pneumoniae)
  • Viral panel for myocarditis 
    •  Adenovirus
    • CMV
    • EBV
    • Enterovirus
    • HHV6
    • Influenza virus A / B 
    • Parvovirus
    • Respiratory Syncytial Virus (RSV)

Lab Hours and Turnaround Times

The laboratory is staffed Monday - Thursday on first shift from 4 am to 3:30 pm, Friday on first shift from 6 am to 3:30 pm, and Monday - Friday on second shift from 3:15 pm to 11 pm, with a limited testing schedule on second shift. Specimens must be in the lab by 6 am to be processed / resulted on first shift and by 3 pm to be processed / resulted on second shift. On Saturday and Sunday the lab is staffed on first shift only from 6:30 am to 3 pm, with a limited testing schedule. Specimens must be in the lab by 6:30 am to be processed / resulted that day. This schedule may vary depending on the need to batch samples or staffing considerations.

For urgent requests you may call the lab at 513-636-9820 to confirm that testing will be done. Any testing outside of this schedule should be coordinated through the laboratory director.  

Collection Requirements

  • When using a swab to collect a sample from any site (nasal / respiratory, lesion, rectal), use a plain, sterile swab with no media. Either the red or green-topped Stuart’s swabs are acceptable. Do not submit swabs that are in any kind of charcoal or gel media.
  • When multiple PCR tests (from the list above) are to be analyzed from whole blood, a single tube of blood (at least 0.5mL) is acceptable. The laboratory does not need a separate tube of blood for each test being ordered.
  • More information about collection requirements for each test can be found in the Clinical Lab Index.