Karnail Singh, PhD, is an assistant professor in the Division of Infectious Diseases at Cincinnati Children's Hospital Medical Center since 2016. He earned his doctorate degree in 1998 from PGIMER, Chandigarh, India.
Dr. Singh’s postdoctoral work in Dr. Covey’s lab at Rutgers University identified important role that RNA binding proteins nucleolin and polypyrimidine tract binding protein (PTB) play by stabilizing CD154 mRNA thereby contributing to its sustained T cell expression. In Dr. Goronzy’s lab in Emory University, Dr. Singh identified K-RAS and B-RAF mediated tolerance defect in T cells from rheumatoid arthritis patients that results in higher basal and post activation p-ERK levels leading to re-calibration of activation threshold in RA T cells. Its therapeutic targeting in an RA mouse model significantly delayed the onset of a much less severe disease. Dr. Singh, along with Dr. Kean, were the first to estimate the size of the peripheral regulatory T cells (Tregs) pool in rhesus macaques. They further showed that rapamycin, in addition to enhancing Tregs’ suppressive activity, also preserves their phenotype and prolongs their persistence following their adoptive transfer.
Singh lab’s current focus is on the development and evaluation of candidate Ebola vaccines. In the absence of any licensed treatment and vaccine for Ebola, mortality rates are often very high. Two experimental vaccines that showed promise in monkeys and are in clinical trials have serious limitations in terms of pre-existing anti-vector immunity or serious side effects. We are developing potential Ebola vaccines on virus-like-particles (VLPs). Glycoproteins from different Ebola species are being expressed on different VLP matrices and their potential to induce cross-protective immunity against different Ebola viruses will be studied. We are also interested in delineating the mechanism(s) how Tregs function and use that knowledge to develop novel molecular and/or cellular therapeutics.
Visit the Singh Lab.
Immunology; infectious diseases; vaccine development and translation; autoimmune diseases.
Assistant Professor, UC Department of Pediatrics