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ACTH Stimulation Test

What is an ACTH Stimulation Test?

An ACTH stimulation test is done to see if the adrenal glands—located on top of each kidney—are able to make the right amount of hormones called cortisol and aldosterone. These hormones help keep blood pressure and blood glucose normal, as well as maintain salt and water balance in the body.

The ACTH stimulation test is done by giving a medication called Cortrosyn and taking measured blood levels. Cortrosyn tells the body to make cortisol. Cortrosyn is given in two different doses; either a low dose or high dose, depending on what your provider orders.

Before the Test

It is important to be off steroids before having this test done. Contact your provider to discuss a plan. Your child can eat normally before the ACTH stimulation test.

  • A low-dose stimulation test is done any time of the day and takes about one hour.
  • A high-dose stimulation test is usually done in the morning and takes about two hours.

Discussing the test ahead of time with your child is helpful.

During the Test

Your child will get an IV (a small, thin tube) placed in their arm. This is used to give the Cortrosyn medication and take the blood samples. Getting the IV may be a little uncomfortable, but it is done quickly.

When the test is complete, the IV is removed. Your child is awake throughout the test.

After the Test

After the testing is completed, your child can go home. Test results are usually available two to three weeks after the test is completed. Your child can return to normal activities and diet after the ACTH stimulation test.

Last Updated 09/2025

Reviewed By Michelle Lawrence, RN
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To learn more, schedule an appointment, or refer a patient, contact the Division of Endocrinology. Contact Us