To use or services or get more information about the Cincinnati Biobank Core, email firstname.lastname@example.org.
For biofluid requests, contact Justin Hogan: email@example.com / 513-803-5166.
For tissue requests, contact Jaime Reuss: firstname.lastname@example.org / 513-803-3338.
To acknowledge the Cincinnati Biobank and Better Outcomes for Children Biorepository in publications, please use the following sentence:
“This research was supported in part by the Cincinnati Children’s Research Foundation and the Cincinnati Biobank, as well as the Better Outcomes for Children Biorepository.”
Substantial institutional commitments have been made for the Cincinnati Biobank Core Facility (CBCF) with over $9 million dollars invested or committed by the Division of Biomedical Informatics, the Division of Pathology, the University of Cincinnati, and the Cincinnati Children’s Research Foundation. Biomedical Informatics purchased biobanking specific software: Biomaterial Tracking and Management (BTM), and Crimson (Daedalus Software, Inc). The Division of Pathology provided start-up funds for the implementation, supply, management and technical staffing, as well as a dedicated Database manager for the software systems. The University of Cincinnati obtained a grant from the State of Ohio to renovate 3000 square feet at the University of Cincinnati’s Reading Road Campus for expanded space and long-term storage. Cincinnati Children’s Research Foundation has committed additional funds to expand and improve the offerings of the CBCF.
The CBCF presently occupies space at Cincinnati Children’s main campus in the research building (R5553) and is dedicated to the processing and storage of samples (DNA, serum, plasma, etc). For processing, the Biobank possesses an AutoGen FLEX Star automated DNA extraction instrument (extract DNA up to 5 mL of whole blood, saliva, or tissue), a Fluidigm EP1 HX SNP analysis instrument, a NanoDrop 2000 spectrophotometer instrument, a Biomek NXp liquid handling system, a Heraeus Megafuge 16R centrifuge, along with two biosafety cabinets and a fume hood. For storage, the Biobank possesses two -80oC and two -20oC upright freezers, and two 4oC refrigerators. Each freezer and refrigerator is on a monitored alarm system (RMMS) and emergency generators supplied by the institution. Office space is available for 6 technicians with networked computer work stations. The institution supports a hospital-wide computer network (both wired and wireless), provides and supports up-to-date computer hardware, and supports a core set of computer software. Tissues are processed and stored within the Department of Pathology. The Pathology lab has additional -80oC and -20oC freezers (with back-ups) and a custom portable -20°C freezer pan (cold table). The same CBCF redundancies exist for Pathology. Office space with networked computer work stations is provided.
The Reading Road Campus facility is leased from the University of Cincinnati and operated by Cincinnati Children's Hospital Medical Center personnel as a Cincinnati Children's facility. For processing, this facility’s lab possesses an AutoGenFlex STAR, two Promega Maxwell MDx automated extraction instruments (DNA or RNA from 300 µL to 2 mL of whole blood, saliva, or PAXgene tubes) with an associated Helix monitored inventory cabinet, a CryoXtract frozen sample aliquotter, a Fotodyne ImageTech gel system, two custom -20°C freezer pans (cold tables), a Sorvall ST-16 centrifuge, an Eppendorf 5430R centrifuge, a Trinean DropSense96 multichannel spectrophotometer instrument, along with a biosafety cabinet. There are also 2 desktop and 1 laptop workstations inside the lab space. For storage, this facility possesses three liquid nitrogen freezers supplied by an exterior 1000 L microbulk storage tank and manifold distribution system, eleven -80oC freezers, thirteen -20oC freezers, and two 4oC refrigerators. The facility has expansion space for up to 13 freezer units in the current configuration as well as an option for an additional 3000 sq. ft. of un-renovated space next door. Office space has workstations for 4 technicians with additional office space in close proximity, as needed. Computers at this facility operate over VPN as if on the Cincinnati Children's Hospital Medical Center main campus. All freezers, refrigerators, room temperature, humidity and oxygen levels are monitored by a Rees monitoring system. Each piece of cold equipment operates on a unique circuit. There is a redundant air handling system and all equipment is included on the facility’s emergency generator system.
For additional information, tailoring the language to your particular grant applications or for letters of support, please contact Laura Gaines at email@example.com.
Download this boilerplate language.