The RFCC at Cincinnati Children’s operates four cell sorters for the simultaneous purification of up to four populations to be used for downstream applications.  If you are a new client, please make an appointment with Monica DeLay to discuss your experimental needs.

For those interested in performing user operated sorts on the Sony SH800S, contact Monica DeLay for information about training.  

Design a panel on one of our instruments using FluoroFinder.

     

Number of Fluorescence PMTs

Instrument Name* Instrument Make/Model Location** Blue (488nm) Red (635nm) Violet (405nm) Yellow-Green (561nm) UV (335nm)
Aria 1 (Ariel) BD FACSAria II R5503 5 3 2   2
Aria 2 (Arnie) BD FACSAria II R5503 2 3 6 5 2
MoFlo Beckman Coulter MoFlo XDP R5529 5 3 2    
SH800S Sony SH800S R5503 4 collinear lasers, 6 Channels

Cell Sorting Information

Location
R5503: FACSAria's, SH800S
R5529: MoFlo XDP

Hours
9 am to 6 pm Monday-Friday

To Schedule (Staff Operated)
513-636-2770 - FACSAria's, SH800S
513-636-2054 - MoFlo XDP

cell sorting

*Click on the instrument name for the configuration.
**BL2 rooms.  Doors must be kept closed at all times and appropriate PPE worn while in the instrument rooms.

Sample tube options include: 1.1 ml tubes (Neptune cat# 2601.S); 12x75 mm Falcon tubes; 15 ml conicals.

Collection options include: 1.5 ml tubes; 12x75 mm Falcon tubes; 15 ml conicals; multiwell plates; slides.

Scheduling

Call 513-636-2770 (FACSAria's) or 513-636-2054 (MoFlo) to schedule a time between 9 am and 6 pm Monday-Friday. We will fill out a scheduling form for each appointment. Be prepared to provide details related to your sort. All appointments, modifications and cancelations are confirmed via email. Click here to view the calendar.

Sample preparation

Read the Cell Sorting Guidelines for information on sample preparation, collection, etc.

Sorting fees

The sorting fees for internal clients can be found on Centerlink. For external clients, please contact us. Clients must inform us of the correct budget number for billing at the time of the sort.

Cancellation / late policy

You must call to cancel your appointment. All cancellations will be confirmed via email to the client. You will be charged for the sort if you cancel less than 24 hours before the scheduled time. For Monday sorts, you must cancel by noon the Friday before. If you are late, you will be charged for the time you scheduled. If your sort time runs over, you will be charged for the additional time you used.

Infectious cell sorting

All of our cell sorters are contained in class II biosafety cabinets for sorting of potentially infectious samples. Contact Monica DeLay for more information on infectious cell sorting.

After your sort

Tell us how we’re doing! After your appointment, fill out a survey to provide feedback on your sort.