Six Cell Sorters for the Simultaneous Purification of Up to Six Populations
The Research Flow Cytometry Facility at Cincinnati Children’s operates six cell sorters for the simultaneous purification of up to six populations to be used for downstream applications. If you are a new client, please make sure you have a Stratocore account, an IBC protocol, and take the required courses (RFCC Course 1 and Course 10 can be found on the training tab). Once courses are completed, users must submit a "Cell Sorter Training/Consultation Form" under the "Request" tab in Stratocore to schedule a consultation.
Users can be trained on the Sonys (SH800S and MA900), FACSArias, FACSymphony S6, and the Bigfoot. Those instruments are available 24/7 for user operated sorts of up to two, four and six populations respectively.
Design a panel on one of our instruments using FluoroFinder.
All our sorters are located in R1.033. In this BL2 room, doors must be kept closed at all times and appropriate PPE worn while in the instrument rooms.
|
Number of Fluorescence PMTs |
Number of cell population |
||||||
| Instrument Name* | Instrument Make/Model | UV (335nm) | Violet (405nm) | Blue (488nm) | Yellow-Green (561nm) | Red (635nm) | |
| Fusion | BD/FACSAria II/Fusion | 2 |
6 |
2 |
5 |
3 |
4 |
| S6 | BD/FACSymphony S6 | 8 | 8 |
6 |
5 | 3 |
6 |
| S6 SE |
BD/FACSymphony S6 SE | 10 |
14 |
9 |
9 |
6 |
6 |
| SH800S | Sony/SH800S | 4 collinear lasers, 6 Channels |
2 |
||||
| MA900 | Sony/MA900 | 2 set of 2 collinear lasers, 12 channels |
4 |
||||
| Bigfoot | ThermoFisher/Bigfoot | 12 |
12 |
7 |
12 |
5 |
6 |
*Click on the instrument name for the configuration.
Collection options include: 1.5 ml tubes; 12x75 mm Falcon tubes; 15 ml conicals; multiwell plates; slides.
Sample preparation
Read the Cell Sorting Guidelines for information on sample preparation, collection, etc.
Sample Transportation to the RFCF
All samples must be placed in sealed capped tubes and in a secondary leak-proof container with absorbent. Please refer to the Transporting Samples to the RFCF document.
Infectious Cell Sorting
All of our cell sorters are contained in class II biosafety cabinets for sorting of potentially infectious samples. Contact us for more information on infectious cell sorting. Please see this list for a non-exhaustive list of BSL2 and BSL2+ samples.
Cancellation Policies and Fees
You need to cancel your appointment via Stratocore. All cancellations will be confirmed via email to the client. Contact us for more information about our policies and fees.
Location and Contact Information
You need to cancel your appointment via Stratocore. All cancellations will be confirmed via email to the client. Contact us for more information about our policies and fees.
Location: Burnet Campus, R Building, 1st Floor, R1.028
Hours: Monday through Friday, 9 am to 6 pm (Sort: 9:30 am to 5:30 pm)
Contact, Fees and Scheduling: For more information about our equipment, services, training and pricing or to begin working with us, please contact us. Reference our billing policies for details about other fees.
