The ImageStreamX imaging flow cytometer combines the visual power of microscopy with the speed and sensitivity of flow cytometry by digitally imaging cells directly in flow. 

Features of our instrument:

  • Lasers - 405 nm, 488 nm, and 642 nm.  Plus a 785 nm laser for Scatter.
    12 Image Channels - Two cameras, each with a brightfield channel leaves up to 10 channels available for fluorescence detection. See Configuration.
  • MultiMag - This option provides 20× and 60× objective lenses in addition to the standard 40× lens for greater imaging flexibility and improved resolution.
  • Extended Depth of Field - EDF™ extended depth of field technology uses a combination of specialized optics and unique image processing algorithms to project all structures within the cell into one crisp plane of focus.
  • Full Color Brightfield - The Full Color Brightfield option provides a full spectrum brightfield light source that allows the ImageStreamX system to replicate the RGB brightfield imagery of a microscope.
    For more information visit the website.


If you would like to be trained on the ImageStreamX, contact Sarah Croswell or Celine Silva Lages. Click here to learn how multispectral imaging in flow can be used to answer specific research questions.


After training, you can schedule time using the CORES and clicking on 'Reserve Core Device'. If you are a non-Cincinnati Children’s employee, email to gain access to the booking system.

Sample Preparation and Protocols

Download the Sample Preparation Guide for information on experimental design and the protocol for cell preparation. Samples are run using 1.5 ml microfuge tubes. Visit their website and register for an online account through the Customer Portal for access to more applications and protocols.


The instrument is located within R5503, which is locked after working hours. If you need access to this room outside of the normal working day, please let us know. This is a BL2 room.


ImageStreamX usage fees for internal clients can be found on Centerlink. For external clients, please contact us. If you would like RFCC staff to run samples for you, contact Celine Silva Lages to discuss your specific needs. 

Technical Support

Need help? We can be reached in R5503 or at 513-636-3575.

Alternatively, call Amnis at 1-800-730-7147, option 2. Support is available 11 am-9 pm M-F.

Data Storage

A project drive has been set up for ImageStreamX data called “RFCC-ImageStream” (Cincinnati Children’s users only). Access will be given after training. During acquisition the data will be stored on the analysis computer. You will then transfer your data to your folder on RFCC-ImageStream where it can be analyzed from any computer running the IDEAS software application.

Request access to one of our project drives at


For analysis of ImageStreamX data, there is a PC workstation next to the instrument with the IDEAS software package. If you would like to install a copy of IDEAS on your PC, you can do so after registering for an account in the Customer Portal. IDEAS will also run on a Mac with ’parallels’ capability.

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