Research Flow Cytometry Facility

Related Resources

• BD Biosciences Multicolor Flow Cytometry Tools − Several tools for flow cytometry education and panel design. 
• Beckman Coulter − A website section dedicated to flow cytometry.
• BioLegend Web tools − Tools for developing panels for multicolor assays.
• ThermoFisher Flow Cytometry - A website section dedicated to flow cytometry.
• Compensation Basics (Mario Roederer)

Fluorochromes/Fluorescent Proteins/Fluorescent Dyes

• Fluorochrome Descriptions
• Fluorochrome Brightness Chart

The Ohio River Valley Cytometry Association (ORVCA) and the Research Flow Cytometry Core (RFCC) organize monthly meetings featuring lectures to educate and inform the local community on applications and products used in cytometry.  Email notifications are sent to users via the Research Flow Core email list and to non-RFCC users via the ORVCA email list.

• BD Biosciences Horizon Guided Panel Solution - Tools for developing panels for multicolor assays.
• BioLegend - Free web-based panel design tool.
• Chromocyte - Free web-based panel design tool, reagent database and forum.
• FluoroFinder - Free panel design software and reagent database. Visit the RFCC page to design panels specific to our instrument configurations.

• BD Biosciences
• BioLegend
• ThermoFisher
• Cytek – For spectral flow cytometry

Each year ORVCA and the RFCC organize the Imaging and Cytometry Research Day to bring education and awareness to the scientific community on cytometric applications and developing technologies.  Please visit the ORVCA website for more details.  For updates, sign up for the ORVCA email list.

• BD FACSymphony A5 validation Panel Results 2023 – Presentation by Ken Quayle
• Maximizing Conventional Cytometry with BD FACSymphony A5 2023 – Presentation by Connor Mahaney
• Standardization of Instrument Setup: monthly High Parameter meeting 2023 - presentation by Sarah Croswell
• New State-of the-Art Technology for Cytometry: Monthly December 2022 ORVCA meeting
• Panel design: monthly High Parameter meeting – preparation by Ken Quayle and Sarah Croswell
• Sorting 101: Annual ORVCA meeting 2022 – Breakout session with Mary Mullen
• Voltage setting: Annual ORVCA meeting 2022 – Breakout session with Ken Quayle
• Doublet on Image stream
• Cell Cycle
• Analysis of Extracellular Vesicles

• Purdue University Cytometry Laboratories - A great resource for all things flow.
• Visit the Signaling-Based Flow and Mass Cytometry Resource by the Nolan Lab at Stanford University
• Visit the Confocal Imaging Core at Cincinnati Children's