Research Flow Cytometry Core

Commercial Flow Cytometry Links and Tools

• FluoroFinder - Free panel design software and reagent database. Visit the RFCC page to design panels specific to our instrument configurations. 
• Fluorish – Free panel design software and reagent database.  Join the ‘Cincinnati Childrens’ core facility to design panels specific to our instrument configurations. 
• Chromocyte - Free web-based panel design tool, reagent database and forum.
• BD Biosciences Multicolor Flow Cytometry Tools − Several tools for flow cytometry education and panel design. 
• Invitrogen’s Fluorescence Tutorials − Five tutorials on the basics of fluorescence, understanding fluorescence spectra, optics, flow cytometry and data analysis.
• BioLegend Webtools − Tools for developing panels for multicolor assays.
•  Affymetrix/eBioscience – Protocols and products for preparing and staining cells for flow cytometry.
• Beckman Coulter − A website section dedicated to flow cytometry.

Spectra Viewers

• Life Technologies
• eBioscience
• BioLegend

Protocols in Flow Cytometry

• Current Protocols in Cytometry (online subscription through the Pratt Library at Cincinnati Children’s)

• OMIPs (Optimized Multicolor Immunofluorescence Panels) – A publication type in Cytometry Part A to communicate complex reagent panels for interrogating phenotypic and functional characteristics of cell populations.

• Publishing Flow Cytometry Data − MIFlowCyt (Minimum Information about a Flow Cytometry experiment) A standard for outlining the minimum information required to report the experimental details of flow cytometry experiments.

Flow Cytometry Reference Books

Basic tutorials for designing a flow cytometry experiment

• Guidelines for Designing an Experiment in Multicolor Flow Cytometry

Fluorochromes/Fluorescent Proteins/Fluorescent Dyes

• Fluorochrome Descriptions
• Fluorochrome Brightness Chart
• Fluorescent Proteins for Flow Cytometry (Albert Einstein Flow Cytometry Facility)
• Compensation Basics (Mario Roederer)

Other Related Cores

• Visit the Shriners Flow Core website for information about satellite sites located at Shriners and University of Cincinnati.
• Purdue University Cytometry Laboratories - A great resource for all things flow.
• Visit the Signaling-Based Flow and Mass Cytometry Resource by the Nolan Lab at Stanford University
• Visit the Confocal Imaging Core at Cincinnati Children's