Shared Core Facilities
NMR-based Metabolomics Core

Experience and Equipment

The NMR-based Metabolomics Core (NBMC) provides state-of-the-art technology in a centralized location accessible to research investigators and clinicians, serving Cincinnati Children’s and University of Cincinnati College of Medicine (UC COM) communities as well as external academic and industry partners.

The NBMC facility, part of the Division of Pathology at Cincinnati Children’s, utilizes a Bruker IVDr 600MHz NMR system that is fully-automated, designed specifically for lipidomics- and metabolomics-based studies of cells, organ tissue (e.g. liver, muscle, intestines, tongue, and tumor), biological fluids (e.g. urine, serum, cerebral spinal fluid, plasma, amniotic fluid and saliva), and exhaled breath collected from human subjects or animal models.

An image showing the core's equipment.

The Bruker IVDr, a top-of-the-line spectrometer, is capable of running most contemporary homonuclear, heteronuclear one and two-dimensional pulse sequences, using pre-designed software, and is equipped with a 96-well SampleJet auto-sampler unit that renders the NMR system fully-automated.

The Bruker spectrometer is equipped with a 5mm triple resonance conventional probe, which allows for routine acquisition of 1D and 2D 1H-1H correlations as well as 1D 13C and 2D 1H-13C correlations.

The NMR instrument can be used for multi-nuclear NMR analysis (31P, 19F, 15N and others), which will benefit your specific study greatly. The usual array of peripheral analytical equipment is available including an electronic balance, pH meters, distillation equipment, bench-top centrifuges, rotary evaporators, a dual SpeedVac concentrator and lyophilizer system, and a cryogenic homogenizer.

Additionally, we have experts that act to facilitate data processing, analysis and interpretation who routinely use advanced software tools, such as MNova, R Program and TopSpin.