Shared Facilities
Translational Metabolomics Facility

Providing Metabolomics Services and Data Analysis to Researchers

The Translational Metabolomics Facility provides state-of-the-art technology in a centralized location accessible to research investigators and clinicians, serving Cincinnati Children’s and University of Cincinnati College of Medicine communities as well as external academic and industry partners.

We provide all NMR-related metabolomics services on human and animal cells, biopsies, and body fluids. Various metabolic biomarkers, related to glycolysis, mitochondrial citric acid cycle, choline, phospholipids and fatty acid metabolism, have recently been reported to play important roles in cancer development and responsiveness to anti-cancer treatment using NMR-based metabolic profiling.

Our facility is fully equipped to study multiple aspects of NMR, including 1H, 13C, and two-dimensional studies. We have performed expanded quantitative metabolic analysis on:

  • Cells extracts/Cell secretions (media)
  • Human and animal tissues and biopsy extracts
  • Body fluids (including blood, plasma, serum, urine, cerebral/pancreatic/prostatic fluids, etc.)

Our facility, part of the Division of Pathology at Cincinnati Children’s, utilizes a Bruker IVDr 600MHz NMR system.

The Bruker spectrometer is fully-automated, designed specifically for lipidomics- and metabolomics-based studies of cells, organ tissue (e.g. liver, muscle, intestines, tongue, and tumor), biological fluids (e.g. urine, serum, cerebral spinal fluid, plasma, amniotic fluid and saliva), and exhaled breath collected from human subjects or animal models.

Learn More About Our Equipment

In addition to our top-of-the-line spectrometer, our experts help facilitate data processing, analysis and interpretation and routinely use advanced software tools, such as MNova, R Program and TopSpin.

Learn More About Our Services

Acknowledgement Statement

This [publication, project, or research] was made possible, in part, using the Cincinnati Children’s Translational Metabolomics Facility [RRID: SCR_022636]. (Optional): We specifically acknowledge the assistance of [name(s) of staff member(s)].