Mass Spectrometry Laboratory

Clinical Mass Spectrometry (CMS) Laboratory

The Clinical Mass Spectrometry (CMS) facility in the Division of Pathology and Laboratory Medicine provides a wide range of assays for endogenous compounds and metabolites, natural and medicinal products, novel drugs under development, therapeutic drug monitoring, detection of drugs of abuse (including opioids), comprehensive lipidomics / metabolomics analyses, and diagnoses of genetic disorders in metabolism. Mass spectrometry is a highly-specific and sensitive technique for the determination of the chemical structure and quantification of compounds based on ionizing and breaking down the molecule into specific fragments. The CMS Facility uses a variety of instrumentation including FAB-MS, GC-MS and LC-MS/MS applications for screening and qualitative and quantitative analyses. Most assays are validated to meet regulatory requirements. The facility operates under GLP standards and is CAP / CLIA accredited. 


CMS clinical services support physicians at Cincinnati Children's and beyond by providing:

  • An international screening program for diagnosis of bile acid synthesis disorders and monitoring response to therapy
  • Trough levels for therapeutic drugs including Sirolimus, Everolimus, Topiramate, Amitriptyline, and Nortriptyline
  • Concentrations of hormones, including thyroid hormone (free-T4) and Testosterone
  • Same-day confirmation of the presence of 46 drugs of abuse and their metabolites, including classes of amphetamines, barbiturates, benzodiazepines, buprenorphine, phencyclidine, cannabinoids, muscle relaxants, cocaine, nicotine, methadone and natural and synthetic opioids
  • Diagnostic testing for genetic disorders of metabolism including organic acids and acyl carnitines

CMS research programs support Primary Investigators from within and outside Cincinnati Children's by:

  • Collaboration, offering over 100 years of collective mass spectrometry expertise 
  • Development of novel methodologies and assay validation
  • Global and targeted metabolomics and lipidomics platforms

Lab Hours and Turnaround times

The laboratory is staffed Monday through Friday from 7:30 am to 5 pm.

Limited testing is available on Saturday and Sunday. Please refer to individual test information sheets for testing schedule. 

For urgent requests, contact the lab at 513-636-4203 for further information on analytical testing. 

Collection Requirements

Please refer to individual test information sheets for collection requirements.

Mass Spectrometry (CMS) Tests Available

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The Clinical Mass Spectrometry (CMS) Laboratory of the Division of Pathology and Laboratory Medicine is located on the main campus of Cincinnati Children’s Hospital, in Research Building Location R, rooms R025 - R034.  

Contact Us

For more information about the Clinical Mass Spectrometry (CMS) Laboratory, call 513-636-4203. You may also reach us by fax at 513-803-5014 or email at

Shipping Address

Clinical Mass Spectrometry Facility
MLC 7019
Department of Pathology and Laboratory Medicine
Cincinnati Children’s Hospital Medical Center
240 Albert Sabin Way
Cincinnati, Ohio 45229