Our current research activities focus on the biology of human epithelial tissues, particularly the epidermis and mucosa, which protect the organism from a hostile environment. As such, we seek to understand gene-environment interactions, including their effects on individuals and their impact on public health.
This research comprises three main areas: 1) directed differentiation of induced pluripotent stem cells into organoids for disease phenotype discovery; 2) studies of intrinsic and environmental stressors of the human epidermis including HPV infection, oncogenic stimuli, metabolic deregulation and radiation; and 3) squamous cell carcinoma and breast cancer development and progression.
In addition to the use of established systems, we have developed and published personalized human models. These include primary patient skin and tumor specimens, as well as derivative cell populations, 3-D spheres and engineered tissues. Genomic, transcriptomic, metabolomic and mechanistic exploration of these systems aims to discover disease drivers and biomarkers. Data obtained are then validated in human cohorts, xenografts and/or genetic mouse models. Our long-term goal is to identify cellular and viral determinants of cancer and to explore their targeting for the purpose of new interventions.
While fundamental basic knowledge discovery remains an important component of our program, we have made key transitions into the realm of translation. Research findings are thus routinely advanced by collaborating clinical teams at Cincinnati Children’s Hospital, the University of Cincinnati, and other national and international Institutions.