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Urine Culture: Female Adolescent

How Do You Do a Urine Culture in a Female Adolescent?

A urine culture is a test that is done to see if there are bacteria (germs) that cause infection in the urine.

A "clean catch" urine specimen (one that has no outside bacteria in it) is necessary for an accurate urine culture.

For the specimen to be "clean," careful cleansing of the area is important.

Needed Supplies

  • Three soapy wipes for cleansing
  • One wet wipe for rinsing
  • One sterile collection container (specimen cup)

Procedure for Urine Collection

  1. Wash your hands well with soap and water.
  2. Place the supplies so they are within reach of the toilet.
  3. Remove the lid from the specimen cup and place it flat side down. Make sure you don't touch the inside of the lid or cup.
  4. Face the wall of the toilet and sit on the front edge of the toilet seat while spreading your legs to straddle the toilet bowl.
  5. Using the fingers of one hand, separate your labia (the folds of the skin around the opening where the urine comes out). Keep this separated for the rest of the procedure.
  6. With the other hand, take one of the soapy wipes and clean one side of the labia, using one stroke from front to back. Discard the wipe.
  7. Take another soapy wipe and clean down the other side. Again, use only one stroke and discard the wipe afterwards.
  8. With the last soapy wipe, clean down the center directly over the meatus (opening where the urine comes out).
  9. Lean your body forward and begin to urinate into the toilet. Place the specimen cup into the urine stream without touching your skin. Remove the cup when it is about half full and finish urinating.
  10. Place the lid tightly on the cup, being careful not to touch the inside of the cup or lid with your fingers.
  11. Wash your hands and leave the cup in the bathroom.

Last Updated 07/2021

Reviewed By Theresa Smith, RN

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