Techniques and Services
Instrumentation
- Confocal laser scanning microscopy
- High-speed single photon confocal laser scanning microscopy
- Multi-photon confocal laser scanning microscopy
- High speed spinning disk confocal with optical reassignment super resolution
Technical Capabilities
- Live imaging of cells, tissues, and animals
- Stochastic Optical Reconstruction Microscopy (STORM) super-resolution
- Total Internal Reflectance Microscopy (TIRF)
- Fluorescence Recovery After Photobleaching (FRAP) microscopy
- Multiplexing (up to 8 channels)
Training
If you are interested in being trained on one the systems complete the Stratocore form:
Training sessions have two parts- the first session is on Microsoft teams and the second session is on the microscope with your prepared sample. Trainings are bimonthly on teams and based on the availability of the staff and instrument. There is no cost for training. See below for steps to initiate training.
CCHMC internal: follow this link to complete the training request form.
External users: send us an email to start the process, cic@cchmc.org.
Advanced Image Analysis
The CIC offers training and assistance with data collected in the CIC. Currently, we have nine image analysis workstations: all have NIS-Elements, and five are designated for Bitplane Imaris. Some have advanced features such as trainable artificial intelligence, denoising, object tracking, deconvolution and batch processing. Training is not required before booking advanced image analysis workstations.