Shared Core Facilities
Research Flow Cytometry Core

Research Flow Cytometry Core

Last update July 7th 2021

The following policies are updates for the RFCC in accordance with the revised Cincinnati Children's Hospital Medical Center COVID policies. For changes in Stratocore, it may take a few days for those to take effect, so please be patient with us. If you have questions, please contact us.

  1. Human samples that did not require fixation prior to the pandemic, will no longer require fixation before running on the analytical cytometers (this will be changed on the form in the near future). To reduce chances of aerosolization of a human sample, tubes with unfixed human samples will need to be capped when vortexing. In addition, adherence to not overfilling waste or running instruments when clogged will reduce the chance of aerosols being generated, so please adhere to these policies.
  2. The RFCC will no longer require approval for Stratocore appointments on the analyzers. You will still need to fill out the form for each appointment for accuracy and attestation of proper biosafety practices. Be aware that appointment information will be spot-checked periodically for accuracy and completeness.
  3. The RFCC will no longer require approval for two people at the same time on any of our cytometers. Please use proper distancing and masking.

 

The Research Flow Cytometry Core (RFCC) is a shared resource laboratory serving the Cincinnati Children's research communities and surrounding institutions by providing a central location for flow cytometry instrumentation, training and education. We offer a number of services for both internal and external scientists.

 

New users need to be trained for their instrument of choice. The training depends on the instrument needed. See Education and Training for more information.

If you have any question, please contact Celine Silva Lages.

Children’s employees: From the lobby of the S building, take an elevator to the 5th floor. Turn left out of the elevator bay and proceed to the R building. Our desks are located in R5503 and the office is in R5564. The instrument rooms are R5503, R5504, R5509, R5510, R5524, R5526, R5529, R5543 and R5087A. From the R building, take an elevator to the 5th floor. Turn left and then right and proceed down the hallway to our area (see room numbers above).

Non-Children’s employees: If you are driving to Cincinnati Children’s, please view directions, maps and information about parking. See building and room numbers above.

When publishing data that is generated using our facility, it is necessary to properly acknowledge us in your publications. See below for example statements and please acknowledge specific staff members as appropriate.

To acknowledge our assistance:
We would like to acknowledge the assistance of the Research Flow Cytometry Core in the Division of Rheumatology at Cincinnati Children’s Hospital Medical Center.

To acknowledge our facility:
All flow cytometric data were acquired using equipment maintained by the Research Flow Cytometry Core in the Division of Rheumatology at Cincinnati Children’s Hospital Medical Center.

For NIH P30 Center Members:
If you are a member of a NIH P30 grant at Cincinnati Children’s, please include ONLY the P30 grant number of the corresponding center by adding the following statement in the acknowledgements ‘supported in part by insert appropriate P30 grant reference(s) here’ (see below).

1) Center for Rheumatic Disease Research (NIH AR070549) or 2) Digestive Health Center (NIDDK P30 DK078392).

For users of the SH800S cell sorter:

For data generated using the SH800S cell sorter please acknowledge the grant used to purchase the instrument by adding the following statement in publications: “supported by NIH S10OD023410”.

For users of the Aurora analyzer:

For data generated using the Aurora analyzer please acknowledge the grant used to purchase the instrument by adding the following statement in publications: “supported by NIH S10OD025045”.

 

General Information

Scheduling:

Analyzers and Sorters: Stratocore

Location

Office: R5503 (Lab desks), R5564 (Main Office)

Analyzer rooms:
R5087A: FACSCantos 1 and 3
R5526: FACSCanto 2
R5524: LSRII and Fortessas 1 and 3
R5543: Fortessa 2
R5503: ImageStreamX Mark II
R5509: Auroras

Sorting Rooms:
R5503: FACSArias and FACSymphony
R5504: MA900 and SH800
R5529: BigFoot

Luminex/ELISA:
R5503

IsoLight:
R5526

Cellometer Vision:
R5503

EnVision:
R5522

GentleMACS Dissociator:
R5510

Analyzer Hours
24 / 7 for those who have been trained by our staff

Sorting Hours
9:30 am to 5:30 pm Monday-Friday (Staff operated)

Contact Us
General information, help and analyzer training:
513-636-3575

Cell sorter rooms:
513-636-2770 - FACSArias and FACSymphony
513-636-2054 - BigFoot
513-517-1466 - SH800 and MA900

Analyzer rooms:
513-636-3332
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