Shared Core Facilities
Research Flow Cytometry Facility

Research Flow Cytometry Facility

The Research Flow Cytometry Facility (RFCF) is a shared resource laboratory serving the Cincinnati Children's research communities and surrounding institutions by providing a central location for flow cytometry instrumentation, training and education. We offer a number of services for both internal and external scientists.

New users need to be trained for their instrument of choice. The training depends on the instrument needed. See Education and Training for more information.

If you have any questions, please contact Celine Silva Lages.

High Parameter Meeting Dates 
9 - 10 am, first and third Fridays in S6.125 or Teams

Date Presenter Subject
1/19/2024 Sandra Andorf, PhD
Biomedical Informatics
Overview of high-parameter flow cytometry data analysis steps
2/2/2024 Ty Troutman, PhD
Allergy and Immunology
Sort on Bigfoot and readout by RNAseq
2/16/2024 Alzebta Godarova, PhD
Subtracting (or not) an extra autofluorescent population in fetal lungs from non-human primates
3/1/2024 Sherry Thornton, PhD
Noel Gibson
Panel Development for analysis of macrophage, monocyte, and T cell populations in systemic juvenile idiopathic arthritis
3/15/2024 Celine Silva Lages, PhD
Guideline for high parameter data
4/5/2024 in S5.125 José Cobeña-Reyes, PhD
Amanda Tursi
Andorf Lab, Division of Biomedical Informatics
Data transformation and cell type naming based on cell ontology
4/19/2024 Tamara Tilburgs, PhD
Zachary Koenig
Immunobiology Graduate Program
Computational analysis workflow of clinical pregnancy samples using high dimensional flow cytometry data

ORVCA Monthly User Meeting Dates 
1-2 PM once a month in S6.125 or Teams

Date Presenter Subject
1/10/2024 Dr. David Morris
BD Biosciences
Demystifying Spectral Cytometry, for the spectrally enabled FACSymphony A5SE and S6SE Sorter
1/24/2024 Eleanor Kincaid
Insights Into the Development of Robust Multicolor Assays
2/7/2024 Ryan Metzler (Virtual Only)
Live demo on how to use the FluoroFinder platform, an essential tool for building flow cytometry panels and designing fluorescent experiments
2/28/2024 Silvia Scaglione (Virtual Only)
A OOC-based approach for 3D cancer tissue culture towards a better understanding of human cancer progression and more predictive drug testing
3/27/2024 Immaculeta Osuji and Jenni Shao
ORVCA 2023 Poster Winner
Eosinophil Regulated Necrosis in Murine Model of Eosinophil Associated Heart Disease

Kruppel-like factor 2+ CD4 T cells avert microbiota induced intestinal inflammation
4/24/2024  Rebecca Bulterma
Staining Cytometry Protocol
5/22/2024 Austin Marshall
Cassandra Clark
Conjugation Kits for Antibodies
6/26/2024 Mary Mullen
Research Flow Cytometry Facility
Panel Design
7/24/2024 Sarah McLeod Multiplexing and expansion in Microscopy

Children’s employees: From the lobby of the S building, take an elevator to the 5th floor. Turn left out of the elevator bay and proceed to the R building. Our desks are located in R5503 and the office is in R5564. The instrument rooms are R5503, R5504, R5509, R5510, R5524, R5526, R5529, R5543 and R5087A. From the R building, take an elevator to the 5th floor. Turn left and then right and proceed down the hallway to our area (see room numbers above).

Non-Children’s employees: If you are driving to Cincinnati Children’s, please view directions, maps and information about parking. See building and room numbers above.

When publishing data that is generated using our facility, it is necessary to properly acknowledge us in your publications. See below for example statements and please acknowledge specific staff members as appropriate.

To acknowledge our assistance:
We would like to acknowledge the assistance of the Research Flow Cytometry Facility in the Division of Rheumatology at Cincinnati Children’s Hospital Medical Center.

To acknowledge our facility:
All flow cytometric data were acquired using equipment maintained by the Research Flow Cytometry Facility in the Division of Rheumatology at Cincinnati Children’s Hospital Medical Center.

For NIH P30 Center Members:
If you are a member of a NIH P30 grant at Cincinnati Children’s, please include ONLY the P30 grant number of the corresponding center by adding the following statement in the acknowledgements ‘supported in part by insert appropriate P30 grant reference(s) here’ (see below).

1) Center for Rheumatic Disease Research (NIH AR070549) or 2) Digestive Health Center (NIDDK P30 DK078392).

For users of the SH800S cell sorter:

For data generated using the SH800S cell sorter please acknowledge the grant used to purchase the instrument by adding the following statement in publications: “supported by NIH S10OD023410”.

For users of the Aurora analyzer:

For data generated using the Aurora analyzer please acknowledge the grant used to purchase the instrument by adding the following statement in publications: “supported by NIH S10OD025045”.


General Information


Analyzers and Sorters: Stratocore


Office: R5503 (Lab desks), R5564 (Main Office)

Analyzer rooms:
R5087A: FACSCantos 1, 2 and 3
R5524: LSRFortessas 1, 2 and 3; and FACSymphony A5
R5503: ImageStreamX Mark II
R5509: Auroras

Sorting Rooms:
R5503: FACSArias and FACSymphony S6
R5504: MA900 and SH800
R5529: BigFoot



Cellometer Vision:


GentleMACS Dissociator:

Analyzer Hours
24 / 7 for those who have been trained by our staff

Sorting Hours
9:30 am to 5:30 pm Monday-Friday (Staff operated)

Contact Us
General information, help and analyzer training:

Cell sorter rooms:
513-636-2770 - FACSArias and FACSymphony S6
513-636-2054 - BigFoot
513-517-1466 - SH800 and MA900

Analyzer rooms: