iPSC Technologies Used in the PSCF
- We have successfully generated iPSCs using multiple methods of reprogramming factor expression in numerous cell types.
- Currently, the PSCF uses a non-integrating recombinant Sendai virus to generate high quality iPSCs from both fibroblasts and non-mobilized peripheral blood cells with high efficiency.
- We have also used a non-integrating, episomal plasmid-based method and an excisable, polycistronic lentiviral vector to generate high quality, integration-free iPSCs.
- Using these methods, we have also successfully generated high-quality iPSCs from disease-specific fibroblasts and peripheral blood mononuclear cells.
- We have successfully generated iPSCs from peripheral blood mononuclear cells, fibroblasts, keratinocytes, lymphoblastoid cell lines (LCLs), CD34 bone marrow and cord blood, nasal and lung epithelial cells, and kidney epithelial cells derived from urine.
iPSC services available in the PSCF
The PSCF is committed to facilitating access to this exciting technology for Cincinnati Children's Hospital Medical Center/UC researchers.
We provide the following iPSC services:
- iPSC generation on a fee-for-service basis
- iPSC characterization and quality control assays
- Training in iPSC generation (Cincinnati Children's/UC investigators only)
Click here for current pricing information.
Download typical iPSC generation flow chart with time line here.
Streamlined patient consenting process
Cincinnati Children's Hospital Medical Center IRB-approved protocol covering any Cincinnati Children's Hospital Medical Center patient for iPSC generation
- Option for parents and other non-Cincinnati Children's Hospital Medical Center patients to be included
- Available to both internal and external investigators
- Remote consenting requires teleconference that must include the physician and participant or legal guardian if minor
- Consented participants agree to all of the following: blood, saliva, skin biopsy (if surgical procedure is occurring) and review of medical records from Cincinnati Children's Hospital Medical Center and outside hospitals.
Contact Misty.Troutt@cchmc.org for details.
Characterization / quality control of iPSCs
Characterizing iPSC lines using a standard series of quality control metrics is critical before investing resources in project-specific iPSC analyses. The PSCF has implemented all the standard assays used for basic iPSC quality assessment.