Confirmation of Genetic Identity
We recommend that STR analysis is used to confirm the identity of iPSCs following generation (e.g. by comparison to donor cells) and during routine culture.
We have successfully performed directed differentiation of iPSC lines into numerous cell types including intestine, neural progenitors and cardiomyocytes. Please see our Differentiation page for details.
Genomic iPSC Characterization
Additional iPSC characterization metrics focusing on genomic analyses (e.g. RNA-seq, micro-RNA, methylation analysis, DNA fingerprinting, copy number variation) are available through the CCHMC Genetic Variation and Gene Discovery Core Facility.
iPSC line banking
iPSC lines generated by the PSCF (for CCHMC/UC investigators only) can be stored securely in liquid nitrogen in the Cincinnati BioBank Core.