Services Offered by PSCF

The PSCF consists of well-equipped tissue culture facilities, access to state of the art microscopy and histology equipment, as well as all the necessary equipment for cellular and molecular analysis of hPSCs. In addition to access to these facilities, we offer the following services to our users:

Routine access to quality tested cells

Induced pluripotent stem cell lines generated from healthy donors (learn more about available lines and quality testing on the FAQ page), Human embryonic stem cells are not available to non-Cincinnati Children's / UC investigators through the PSCF.

iPSC generation and characterization services

  • Human iPSC generation from fibroblasts and blood
  • Streamlined patient consenting process

Please see the iPSC section of the website for more details.

CRISPR-Cas9 mediated genome editing of hPSCs

Please see the genome-editing section of the website for more details

Hands-on training services

Please see the training section of the website for more details

HESC/iPSC characterization and quality control assays

  • Pluripotency marker analysis
  • Functional pluripotency testing (Scorecard assay)
  • Karyotype analysis
  • Identity testing by STR analysis
  • Mycoplasma testing

Supplies for hESC Culture and Analysis

All routine supplies for hPSC culture are available from the PSCF (only available to Cincinnati Children's / UC investigators)

Consulting

  • Experimental design
  • Data analysis
  • Manuscript and grant preparation
  • Letters of support

Acknowledging the PSCF in your manuscripts and presentations

Investigators receiving services/cell lines from the PSCF are required to acknowledge the facility in publications and presentations arising from the work. The following are examples of an appropriate acknowledgments that could be used in publications: “This work was supported by the Cincinnati Children's Hospital Medical Center Pluripotent Stem Cell Facility” or "The iPSC lines used in this study were generated by the Cincinnati Children's Pluripotent Stem Cell Facility".