Options for Interacting with PSCF

Depending on your requirements, the PSCF operates on a fee-for-service basis or on a more collaborative basis. Preferences should be discussed with the PSCF director before beginning your project. 

  • Fee-for service - investigators using this option will be billed for all cells/reagents and all associated PSCF employee labor costs
  • Collaboration -  PSCF staff will be more heavily involved in design and execution of experiments, data analysis and interpretation, manuscript generation etc.  Investigators using this option will typically only be billed for cells/reagents. This option requires approval of proposed research by the PSCF director and agreement on salary support  

Services Offered by PSCF

The PSCF consists of well-equipped tissue culture facilities, access to state of the art microscopy and histology equipment, as well as all the necessary equipment for cellular and molecular analysis of hPSCs. In addition to access to these facilities, we offer the following services to our users:

Routine access to quality tested cells

Human embryonic stem cell and induced pluripotent stem cell lines (learn more about available lines and quality testing on the FAQ page)

iPSC generation and characterization services

Please see the iPSC section of the website for more details

CRISPR-Cas9 mediated genome editing of hPSCs

Please see the genome-editing section of the website for more details

Hands-on training services

Please see the training section of the website for more details 

HESC/iPSC characterization and quality control assays
  • Pluripotency marker analysis
  • Functional pluripotency testing (EB assay, teratoma assay)
  • Karyotype analysis
  • Mycoplasma testing
Supplies for hESC Culture and Analysis

All routine supplies for hPSC culture are available from the PSCF

Consulting
  • Experimental design
  • Data analysis
  • Manuscript and grant preparation
  • Letters of support

Acknowledging the PSCF in your manuscripts and presentations

Investigators receiving services/cell lines from the PSCF are required to acknowledge the facility in publications and presentations arising from the work. The following are examples of an appropriate acknowledgments that could be used in publications: “This work was supported by the Cincinnati Children's Hospital Medical Center Pluripotent Stem Cell Facility” or "The iPSC lines used in this study were generated by the Cincinnati Children's Pluripotent Stem Cell Facility".