Brain tsunami spreading across a mouse brain recorded simultaneously in 3 different optical channels.
A miniature microscope with a mount that allows rodents to be imaged.
Watching the Behaving Brain
In the field of functional neurosurgery we know that the best way to understand where seizures are coming from is to monitor them in the most “natural” way possible. This means observing patients for days or weeks with electrodes implanted in their brains and waiting for spontaneous seizure events to occur so they can be captured by our monitors. We correlate that information with videos of the patients to understand which electrical waveforms match up with certain exhibited clinical features (semiology). We hypothesize that some of the data on seizures and spreading depolarizations from slice preparations or even anesthetized in vivo models may not precisely represent spontaneous events or triggered events with anesthetic on board.
Using miniature head-mounted microscopes we aim to study seizure and spreading depolarization events in awake, behaving animals. Understanding the technical challenges of these types of recordings as well as the potential differences compared to anesthetized data is key in moving toward implementation of optical monitoring systems in clinical practice.
Candi LaSarge, PhD, and Steve Danzer, PhD.