The rheumatic diseases are autoimmune processes, which by definition involve aberrations in the immune system. Studies investigating various aspects of immune responses are therefore of potential relevance to furthering the understanding of the pathophysiology of the rheumatic diseases and developing new therapeutic approaches. This includes studies of the immune cells in rheumatic diseases such as JRA, RA, Kawasaki disease and dermatomyositis, as well as studies of the basic biology of cells comprising the immune system, such as macrophages, monocytes, lymphocytes and dendritic cells.
Technology provided by the core is fundamental to the study of the biology of cells of the immune system. The core allows analysis of cell phenotypes at a single cell level as well as for isolation of specific subpopulations of immune cells for further study. Understanding the pathobiology of these diseases often requires dissecting out contributions of specific cell subpopulations. The SCPC (Single Cell Phenotyping Core) will provide technical expertise, assistance and equipment to investigators in the Research Base of the Cincinnati Rheumatic Disease Core Center (CRDCC) whose studies require single cell analysis. The SCPC will provide training in the proper use and interpretation of a number of immunophenotyping methodologies, including flow cytometry and single cell gene expression. Expertise and assistance will be provided by Core personnel in the following areas:
- Cell sorting
- Cell surface phenotyping
- Intracellular cytokine detection
- Gene expression reporter analysis
- Cell proliferation analysis
- Cell cycle and DNA content analysis
- Apoptosis analysis
- Intracellular calcium mobilization analysis
- Tetramer staining of antigen-specific T cells
- RNA detection by flow cytometry
- Cell signalling analysis
- CRISPR genome-edited cell identification and sorting
- Imaging cytometry
- Single cell manipulation and gene expression analysis
In addition, the SCPC will facilitate acquisition, analysis and storage of immunophenotyping data, maintain standards and quality control for immunophenotyping procedures, and assist in the application and development of new techniques.
Contact Sherry Thornton, PhD for more information or to use our services.