Beginning July 1, 2017, all viral PCR analysis will be transferred to the Molecular Pathology (PCR) Laboratory at Cincinnati Children's under the direction of David Witte, MD. The Molecular Pathology Lab has offered viral PCR services for over 20 years, including the heart PCR studies.
The Heart Institute Diagnostic Laboratory (HIDL) currently offers molecular confirmation of viral infection. PCR analysis for viral genome is available for nine viruses identified as causing myocarditis. Viral infection of the heart can lead to acute or chronic cardiac dysfunction or organ rejection in patients with heart transplantation. The detection of viral genomic nucleic acid sequences by PCR provides a highly sensitive and specific method of diagnosis of viral infection. PCR is extremely sensitive and rapid, and when associated with other molecular biological methods, can facilitate an accurate description of the state of the infecting virus at the molecular level.
PCR Analysis is available at Cincinnati Children’s for the following viruses. The Heart Institute Diagnostic Laboratory also offers a discounted rate for analysis of all nine viruses.
- Epstein-Barr virus
- Human Herpes Virus 6
- Influenza A virus
- Respiratory syncytial virus (RSV)
- Hepatitis C virus (HCV)
- Viral panel (all viruses listed above)
- Download the Viral PCR requisition form.
Send samples Monday through Thursday overnight to:
The Heart Institute Diagnostic Laboratory
Cincinnati Children’s Hospital Medical Center
240 Albert Sabin Way, S4.381
Cincinnati, OH 45229
Samples in RNAlater solution preferred.
RNAlater solution: The sample should be cut into slices no more than 0.5cm across, if necessary, and immediately placed in a minimum of 10 sample volumes of RNAlater solution (Qiagen Cat. #: 76154 or 76163).