Bio-Imaging and Analysis Facility

Equipment

Nikon FN1 Upright Multiphoton Confocal Laser Scanning Microscope

Special Features

• Single harmonic generation for collagen detection.

Stage
Fully encoded scanning XY motorized stage for multipoint and tile scanning. High-speed piezo Z movement with 600 µm range.

Laser Lines
Multiphoton Coherent Chameleon II TiSapphire IR laser, tuneable from 700-1040 nm. Single photon imaging with 405, 488, 561 and 640 nm solid state diode lasers.

Scanner
Dual scanner: resonant and galvanometric.

Software
NIS Elements v 5.41.

Confocal Spinning Disk Microscope Yokogawa SoRa W1, Dual Camera System

Special Features

• High-speed wide-field and diffraction-limited confocal imaging with dual 95% QE cameras.
• Super-resolution using optical reassignment to 120 nm.
• Photoactivation / FRAP with 405 nm laser.
• Software controlled automatic Water Immersion Dispenser (WID) for time-lapse imaging.

Stage
Fully encoded scanning XY motorized stage for multipoint and tile scanning. High-speed piezo Z movement with 600 µm range.

Laser Lines
Multi-channel excitation with 405, 445, 488, 514, 561, 594 and 640 nm solid state diode lasers.

Scanner
Dual scanner: resonant and galvanometric.

Software
NIS Elements v 5.41.

Nikon AXR Upright Confocal Laser Scanning Microscope

Special Features

• Autosignal.ai.
• Faraday cage for electrophysiology experiments.
• Dipping lenses, including 20X Glycerol specialty dipping lens for improved resolution due to RI matching.
• 25 mm field of view.

Stage
Fully encoded scanning XY motorized stage for multipoint and tile scanning. High-speed piezo Z movement with 400 µm range.

Laser Lines
Multi-channel excitation with 405, 445, 488, 514, 567, 594 and 640 and 730 nm solid state diode lasers.

Scanner
Dual scanner: resonant and galvanometric (8K scan area).

Software
NIS Elements v 5.41.

Nikon AXR Inverted Confocal Laser Scanning Microscope (2 systems)

Special Features

• Heated stage inserts with C02 and humidity for cultured embryo/cell work.
• Software controlled automatic Water Immersion Dispenser (WID) for time-lapse imaging.
• Autosignal.ai.
• 25 mm field of view. 
• AxR #2 features an NSPARC detector Image Scanning Microscopy (ISM) SPAD spatial array super resolution microscope with resolution to ~100nm. 

Stage
Fully encoded scanning XY motorized stage for multipoint and tile scanning. High-speed piezo Z movement with 600 µm range.

Scanner
Dual scanner: resonant and galvanometric (8K scan area).

Software
NIS Elements v 5.41.

Nikon A1R Inverted LUNV Confocal Laser Scanning Microscope

Special Features

• Heated stage inserts with C02 and humidity for cultured embryo/cell work. 
• STORM super resolution and TIRF with EMCCD monochrome camera. 
• FRAP capabilities. 

Stage
Fully encoded scanning XY motorized stage for multipoint and tile scanning. High-speed piezo Z movement with 200 µm range. 

Laser Lines
Advanced diode-based laser launch with 405, 445, 488, 514, 561 and 640 nm lasers. 

Scanner
Dual scanner: resonant and galvanometric (4K scan area).

Software
NIS Elements v 5.41. 

Nikon A1R Inverted Confocal Laser Scanning Microscope (2 systems)

Stage
Fully encoded scanning XY motorized stage for multipoint and tile scanning. High-speed piezo Z movement with 100 µm range.

Laser Lines
Solid state diode-based laser launch with 405nm, 488nm, 514nm, 561nm, and 640nm lasers.

Scanner
Dual scanner: resonant and galvanometric (4K scan area).

Software
NIS Elements v 5.41. 

Nikon A1 Inverted LUNV Confocal Laser Scanning Microscope

Stage
Fully encoded scanning XY motorized stage for multipoint and tile scanning.

Laser Lines
Advanced diode-based laser launch with 405, 445, 488, 514, 561 and 640 nm lasers.

Scanner
Galvanometric scanner (4K scan area).

Software
NIS Elements v 5.41. 

Nikon A1 Inverted Confocal Laser Scanning Microscope

Stage
Fully encoded scanning XY motorized stage for multipoint and tile scanning.

Laser Lines
Diode-based laser launch with 405, 488, 561 and 640 nm lasers.

Scanner
Galvanometric scanner (4K scan area).

Software
NIS Elements v 5.41.

Bruker LCS SPIM Light Sheet

Special Features

• Dual sided illumination enables fast acquisition speed combined with high resolution imaging perfect for thick, whole-mount cleared samples 
• 1 high-speed Hamamatsu Orca Flash sCMOS cameras (2048 x2048 pixels)

Stage
High precision, fully encoded scanning XY motorized stage for multipoint and tile scanning

Laser Lines
Up to 6 (405, 488, 561. 642, 785)

Software
Luxendo

Bruker TruLive SPIM Light Sheet

Special Features

• Environmental chamber with incubation for live organoids, explants, and zebrafish embryos.
• Dual sided illumination immersion objectives for a sheet thickness of ~1um.
• 2 high-speed Hamamatsu Orca Flash sCMOS cameras with wavelength switcher.

Stage
High precision XYZ translation for multipoint imaging and tile scanning.

Laser Lines
405nm, 488nm, 514nm, 561nm, 640nm

Software
Luxendo

Nikon Ni-E upright motorized fluorescence microscopes (2 systems)

Stage
Fully encoded scanning XY motorized stage for multipoint and tile scanning.

Light Source
LED illumination for fluorescence between 405 and 730 nm.

Camera
Color and monochrome cameras.

Software
NIS Elements v 5.41. 

Nikon Ti-E/Ti-2 SpectraX inverted motorized fluorescence microscopes (2 systems)

Special Features

• Heated stage inserts with C02 and humidity for cultured embryo/cell work.
• Direct triggering of LED source via camera signal for high-speed acquisition.

Stage
Fully encoded scanning XY motorized stage for multipoint and tile scanning.

Light Source
LED illumination for fluorescence between 405nm and 730nm Camera: Color and monochrome cameras.

Software
NIS Elements v 5.41.