Rothenberg Lab
Rothenberg CURED Lab

Protocols

Reproducibility is a key element of science and validates findings of a single study. Some of the protocols and methods used by the Rothenberg CURED Lab are provided here.

Molecular Biology Protocols

Northern Protocol

Southern Protocol

Reverse Transcription Protocol

RNA Isolation Protocol

FACs Analysis Protocol-Eric's

PhosphoFlow FACs

Primary Biopsy Culture Protocol

Chemotaxis Assays

Immunoprecipitations from Eosinophils

Eosinophil Isolation from Human Blood

Mouse Protocols

Tail DNA Protocol

Tissue Isolation From Intestine

Intranasal Aspergillus Protocol

Raft Culture Protocol

Mouse Primary Fibroblast Tissue Culture

Bronchoalveolar Lavage Fluid Collection and Analysis

Cytokine Induced Airway Inflammation

DSS Induced Colitis

Glucose Tolerance and Measurements

OVA Induced Intestinal Anaphylaxis

Histologic Protocols

Analysis of Epithelial Cell Proliferation

Assessment of Angiogenesis

Esophageal Epithelial Thickness and Collagen Quantitation

Intestinal Histopathic Examination

Quantification of Tissue Eosinophils

Rothenberg LabRothenberg CURED Lab

Protocols

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Rothenberg Lab
Rothenberg CURED Lab