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Reproducibility is a key element of science and validates findings of a single study. Some of the protocols and methods used by the Rothenberg CURED Lab are provided here.
Northern Protocol
Southern Protocol
Reverse Transcription Protocol
RNA Isolation Protocol
FACs Analysis Protocol-Eric's
PhosphoFlow FACs
Primary Biopsy Culture Protocol
Chemotaxis Assays
Immunoprecipitations from Eosinophils
Eosinophil Isolation from Human Blood
Tail DNA Protocol
Tissue Isolation From Intestine
Intranasal Aspergillus Protocol
Raft Culture Protocol
Mouse Primary Fibroblast Tissue Culture
Bronchoalveolar Lavage Fluid Collection and Analysis
Cytokine Induced Airway Inflammation
DSS Induced Colitis
Glucose Tolerance and Measurements
OVA Induced Intestinal Anaphylaxis
Analysis of Epithelial Cell Proliferation
Assessment of Angiogenesis
Esophageal Epithelial Thickness and Collagen Quantitation
Intestinal Histopathic Examination
Quantification of Tissue Eosinophils
