Allergy and Immunology
Rothenberg CURED Lab

Rothenberg CURED Research Lab

Comprehensive Research on Mechanisms of Allergic Response

The Rothenberg CURED Research Laboratory, supported by the Campaign Urging Research for Eosinophilic Diseases (CURED), is focused on elucidating the mechanisms of allergic responses, especially in mucosal tissues such as the gastrointestinal tract and lung. The goal of our research is to identify mechanisms of allergic inflammation with the aim of developing and testing novel diagnostics and pharmaceutical targets for the treatment and cure of patients with a variety of allergic diseases, especially eosinophilic gastrointestinal disorders (EGID) [e.g. eosinophilic esophagitis (EoE)], hypereosinophilic syndrome (HES), asthma and food allergies.

Bench to Bedside and Back

We have identified and biologically characterized several critical pathways that regulate allergic responses. Our research integrates multidisciplinary basic studies, with particular emphasis on modern genetic approaches, stem cell research, biochemistry, cell biology, informatics, translational research employing novel in vivo and ex vivo systems developed by our laboratory (to access datasets, visit EGIDExpress Data Sharing in Current Projects), and clinical studies designed to test proof-of-concept theories and novel therapeutics directly in humans.

Focus on Eosinophils: Homeostasis and Disease

Eosinophils have been considered end-stage cells involved in host protection against parasites. However, numerous lines of evidence have now changed this perspective by showing that eosinophils are pleiotropic multifunctional leukocytes involved in initiation and propagation of diverse inflammatory responses, as well as modulators of innate and adaptive immunity. For example, the Rothenberg laboratory has found that intestinal eosinophils regulate the production of secretory immunoglobulin A (IgA) and regulate intestinal commensal flora.

We are examining new views on the role of eosinophils in homeostatic function, including developmental biology and innate and adaptive immunity (as well as interaction with mast cells, B cells and T cells). We are studying the molecular steps involved in eosinophil development and trafficking, with special attention to the important role of eosinophil-selective cytokines such as interleukin 5 (IL-5), the eotaxin subfamily of chemokines, IL-13 and epithelial gene products.

We are investigating the role of eosinophils in disease processes including infections, asthma and gastrointestinal disorders. We are studying the consequences of genetically engineered eosinophil-deficient mice and eosinophil depletion in humans ("human eosinophil knockouts"). Genetic approaches to understanding eosinophil-associated human diseases are a focus area. Why patients develop allergic disorders, focused on genetic and environmental factors and their interactions, are priority research topics. Finally, we are pursuing strategies for diagnostics and patient-reported outcomes and targeted therapeutic intervention in allergic diseases with a focus on eosinophil-mediated diseases.

Best of Our Research

Focus on Epithelial Cells in Immunity

Epithelial cells are the first line of defense against foreign antigens. We are researching the role of epithelial cells as an innate immune organ. Studies focus on stem cell biology, differentiation and repair. Recent findings include the 1) role of anti-proteases in maintaining homeostasis (e.g., Science Translational Medicine 2020) and 2) identification and role of RipIL33, an allergen sensor that triggers IL-33 maturation and release (Nature Immunology 2021).


EGIDExpress–Gateway for Data Sharing

Groundbreaking Research

Research effort by Cincinnati Children’s investigators helps enable first new class of Asthma drugs in over a decade


Dr. Rothenberg leads within and outside of Cincinnati Children’s. He has greatly furthered the field of allergy and immunology, as evidenced by his extensive publications (over 500) and receipt of an NIH MERIT Award in recognition and support of his research. Rothenberg is the principal investigator of the Consortium of Eosinophilic Gastrointestinal Disease Researchers (CEGIR) and is the director of Cincinnati Children’s Division of Allergy and Immunology and the Cincinnati Center for Eosinophilic Disorders (CCED).

Video Resources

The Rothenberg CURED Research Lab is an active member of research, professional and patient advocacy communities for allergic and eosinophilic conditions. Learn more in our video library.


The Consortium of Eosinophilic Gastrointestinal Disease Researchers (CEGIR) is dedicated to improving the lives of individuals with eosinophilic gastrointestinal disorders through innovative research, clinical expertise and education via collaborations between scientists, health care providers, patients, and professional organizations. CEGIR is part of the Rare Diseases Clinical Research Network (RDCRN) and has a contact registry. Consider joining today. Visit the CEGIR website.