Wells Lab

Publications

Poling, HM; Singh, A; Kongsomros, S; Sheth, M; Krutko, M; Reza, AA; Srivastava, K; Wells, JM; Helmrath, MA; Esfandiari, L. Promoting Human Intestinal Organoid Formation and Stimulation Using Piezoelectric Nanofiber Matrices. Tissue Engineering - Part A.. 2026; 32(17-18):604-615.

Brassard, JA; Wells, JM. Designing complex organoids through developmental principles. Cell Stem Cell. 2026; 33(7):1084-1097.

Zavros, Y; Little, AS; Santagata, S; Wells, JM; Mayhew, C; Kim, AH; Zada, G; Chakrabarti, J; Eschbacher, JM; Yuen, KCJ; Pandey, R; Mochel, JP; Allenspach, K; Jordan, G. SUN-056 The National Biorepository and Resource for Pituitary Neuroendocrine Tumor Translational Research (BioPitNeT) Journal of the Endocrine Society. 2025; 9(Supplement_1):bvaf149.1641.

Yang, K; Spitzer, H; Sterr, M; Hrovatin, K; De La O, S; Zhang, X; Setyono, ESA; Ud-Dean, M; Walzthoeni, T; Flisikowski, K; Wolf, E; Kemter, E; Theis, FJ; Lickert, H. A multimodal cross-species comparison of pancreas development. Nature Communications. 2025; 16(1):9355.

Miao, Y; Pek, NM; Tan, C; Jiang, C; Yu, Z; Iwasawa, K; Shi, M; Kechele, DO; Sundaram, N; Pastrana-Gomez, V; Zorn, AM; Chen, Y-W; Guo, M; Gu, M. Co-development of mesoderm and endoderm enables organotypic vascularization in lung and gut organoids. Cell. 2025; 188(16):4295-4313.e27.

Jurickova, I; Dreskin, BW; Angerman, E; Bonkowski, E; Nguyen, J; Villarreal, R; Tominaga, K; Iwasawa, K; Braun, T; Takebe, T; Helmrath, MA; Haberman, Y; Wells, JM; Denson, LA. Eicosatetraynoic Acid Regulates Profibrotic Pathways in an Induced Pluripotent Stem Cell-Derived Macrophage-Human Intestinal Organoid Model of Crohn's Disease. Journal of Crohn's and Colitis. 2025; 19(2).

Tominaga, K; Kechele, DO; Sanchez, JG; Vales, S; Jurickova, I; Roman, L; Asai, A; Enriquez, JR; Mccauley, HA; Kishimoto, K; Zorn, AM; Helmrath, MA; Denson, LA; Wells, JM. Deriving Human Intestinal Organoids with Functional Tissue-Resident Macrophages All From Pluripotent Stem Cells. Cellular and Molecular Gastroenterology and Hepatology (CMGH). 2025; 19(4):101444.

Dunbar, KJ; Lee, S-H; Won, Y; Esquea, EM; Yang, JY; Kechele, DO; Ryeom, S; Abrams, JA; Mckeon, FD; Wells, JM; Que, J; Wang, TC; Rustgi, AK; Goldenring, JR. A Cell Marker Atlas to Distinguish Metaplastic Transitions in Human Esophagus and Stomach. Cellular and Molecular Gastroenterology and Hepatology (CMGH). 2025; 19(12):101611.

Tornabene, P; Wells, JM. Exploring optimal protocols for generating and preserving glucose-responsive insulin-secreting progenitor cells derived from human pluripotent stem cells. European Journal of Cell Biology. 2024; 103(4):151464.

Poling, HM; Sundaram, N; Fisher, GW; Singh, A; Shiley, JR; Nattamai, K; Govindarajah, V; Cortez, AR; Krutko, MO; Ménoret, S; Mayhew, CN; Takebe, T; Mahe, MM; Helmrath, MA. Human pluripotent stem cell-derived organoids repair damaged bowel in vivo. Cell Stem Cell. 2024; 31(10):1513-1523.e7.

Kang, S-Y; Kimura, M; Shrestha, S; Lewis, P; Lee, S; Cai, Y; Joshi, P; Acharya, P; Liu, J; Yang, Y; Alsberg, E; Wells, JM; Takebe, T; Lee, M-Y. A Pillar and Perfusion Plate Platform for Robust Human Organoid Culture and Analysis. Advanced healthcare materials. 2024; 13(21):e2302502.

Sanchez, JG; Rankin, S; Paul, E; Mccauley, HA; Kechele, DO; Enriquez, JR; Jones, N-H; Greeley, SAW; Letourneau-Frieberg, L; Zorn, AM; Krishnamurthy, M; Wells, JM. RFX6 regulates human intestinal patterning and function upstream of PDX1. Development. 2024; 151(9).

Gilbert, PM; Hofmann, S; Ng, H-H; Vankelecom, H; Wells, JM. Organoids in endocrine and metabolic research: current and emerging applications. Nature Reviews Endocrinology. 2024; 20(4):195-201.

Rivron, NC; Martinez-Arias, A; Sermon, K; Mummery, C; Schöler, HR; Wells, J; Nichols, J; Hadjantonakis, A-K; Lancaster, MA; Moris, N; Fu, J; Sturmey, RG; Niakan, K; Rossant, J; Kato, K. Changing the public perception of human embryology. Nature Cell Biology. 2023; 25(12):1717-1719.

Múnera, JO; Kechele, DO; Bouffi, C; Qu, N; Jing, R; Maity, P; Enriquez, JR; Han, L; Campbell, I; Mahe, MM; Hu, Y-C; Takebe, T; Helmrath, MA; Wells, JM. Development of functional resident macrophages in human pluripotent stem cell-derived colonic organoids and human fetal colon. Cell Stem Cell. 2023; 30(11):1434-1451.e9.

Bouffi, C; Wikenheiser-Brokamp, KA; Chaturvedi, P; Sundaram, N; Goddard, GR; Wunderlich, M; Brown, NE; Staab, JF; Latanich, R; Zachos, NC; Mulloy, JC; Zorn, AM; Wells, JM; Helmrath, MA. In vivo development of immune tissue in human intestinal organoids transplanted into humanized mice. Nature Biotechnology. 2023; 41(6):824-831.

Marek-Yagel, D; Stenke, E; Pode-Shakked, B; Dunne, C; Crushell, E; Bryce-Smith, A; Mcdermott, M; O'sullivan, MJ; Veber, A; Krishnamurthy, M; Wells, JM; Anikster, Y; Bourke, B. Nonsense mutation in the novel PERCC1 gene as a genetic cause of congenital diarrhea and enteropathy. Human Genetics. 2023; 142(5):691-696.

Singh, A; Poling, HM; Chaturvedi, P; Thorner, K; Sundaram, N; Kechele, DO; Childs, CJ; Mccauley, HA; Fisher, GW; Brown, NE; Spence, JR; Wells, JM; Helmrath, MA. Transplanted human intestinal organoids: a resource for modeling human intestinal development. Development. 2023; 150(9).

Mccauley, HA; Riedman, AM; Enriquez, JR; Zhang, X; Watanabe-Chailland, M; Sanchez, JG; Kechele, DO; Paul, EF; Riley, K; Burger, C; Lang, RA; Wells, JM. Enteroendocrine Cells Protect the Stem Cell Niche by Regulating Crypt Metabolism in Response to Nutrients. Cellular and Molecular Gastroenterology and Hepatology (CMGH). 2023; 15(6):1293-1310.

Rosselot, AE; Park, M; Kim, M; Matsu-Ura, T; Wu, G; Flores, DE; Subramanian, KR; Lee, S; Sundaram, N; Broda, TR; Wells, JM; Hogenesch, JB; Moore, SR; Hong, CI. Ontogeny and function of the circadian clock in intestinal organoids. EMBO Journal. 2022; 41(2):e106973.

Cable, J; Lutolf, MP; Fu, J; Park, SE; Apostolou, A; Chen, S; Song, CJ; Spence, JR; Liberali, P; Lancaster, M; Gouti, M; Hombrink, P; Quadrato, G; Urenda, J-P. Organoids as tools for fundamental discovery and translation-a Keystone Symposia report. Annals of the New York Academy of Sciences. 2022; 1518(1):196-208.

Katz, AE; Yang, M-L; Levin, MG; Tcheandjieu, C; Mathis, M; Hunker, K; Blackburn, S; Eliason, JL; Coleman, DM; Fendrikova-Mahlay, N; Li, JZ; Damrauer, SM; Stanley, JC; Ganesh, SK. Fibromuscular Dysplasia and Abdominal Aortic Aneurysms Are Dimorphic Sex-Specific Diseases With Shared Complex Genetic Architecture. Circulation. Genomic and precision medicine. 2022; 15(6):e003496.

Enriquez, JR; Mccauley, HA; Zhang, KX; Sanchez, JG; Kalin, GT; Lang, RA; Wells, JM. A dietary change to a high-fat diet initiates a rapid adaptation of the intestine. Cell reports. 2022; 41(7):111641.

Wells, JM; Guo, Z. In preprints: humans, the new model organism. Development. 2022; 149(22).

Kishimoto, K; Iwasawa, K; Sorel, A; Ferran-Heredia, C; Han, L; Morimoto, M; Wells, JM; Takebe, T; Zorn, AM. Directed differentiation of human pluripotent stem cells into diverse organ-specific mesenchyme of the digestive and respiratory systems. Nature Protocols. 2022; 17(11):2699-2719.

Wells, JM. A renaissance for developmental biology driven by new in vitro platforms. Development. 2022; 149(20).

Krishnamurthy, M; Kechele, DO; Broda, T; Zhang, X; Enriquez, JR; Mccauley, HA; Sanchez, JG; Mccracken, K; Palermo, J; Bernieh, A; Neef, HC; Heider, A; Dauber, A; Wells, JM. Using Human Induced Pluripotent Stem Cell-Derived Organoids to Identify New Pathologies in Patients With PDX1 Mutations. Gastroenterology. 2022; 163(4):1053-1063.e7.

Pitstick, AL; Poling, HM; Sundaram, N; Lewis, PL; Kechele, DO; Sanchez, JG; Scott, MA; Broda, TR; Helmrath, MA; Wells, JM; Mayhew, CN. Aggregation of cryopreserved mid-hindgut endoderm for more reliable and reproducible hPSC-derived small intestinal organoid generation. Stem Cell Reports. 2022; 17(8):1889-1902.

Jurickova, I; Bonkowski, E; Angerman, E; Novak, E; Huron, A; Akers, G; Iwasawa, K; Braun, T; Hadar, R; Hooker, M; Takebe, T; Mollen, KP; Haberman, Y; Denson, LA. Eicosatetraynoic Acid and Butyrate Regulate Human Intestinal Organoid Mitochondrial and Extracellular Matrix Pathways Implicated in Crohn's Disease Strictures. Inflammatory Bowel Diseases. 2022; 28(7):988-1003.

Sanchez, JG; Enriquez, JR; Wells, JM. Enteroendocrine cell differentiation and function in the intestine. Current Opinion in Endocrinology, Diabetes and Obesity. 2022; 29(2):169-176.

Eicher, AK; Kechele, DO; Sundaram, N; Berns, HM; Poling, HM; Haines, LE; Sanchez, JG; Kishimoto, K; Krishnamurthy, M; Han, L; Zorn, AM; Helmrath, MA; Wells, JM. Functional human gastrointestinal organoids can be engineered from three primary germ layers derived separately from pluripotent stem cells. Cell Stem Cell. 2022; 29(1):36-51.e6.