Wells Lab

Publications

Tornabene, P; Wells, JM. Exploring optimal protocols for generating and preserving glucose-responsive insulin-secreting progenitor cells derived from human pluripotent stem cells. European Journal of Cell Biology. 2024; 103(4):151464.

Kang, S-Y; Kimura, M; Shrestha, S; Lewis, P; Lee, S; Cai, Y; Joshi, P; Acharya, P; Liu, J; Yang, Y; Alsberg, E; Wells, JM; Takebe, T; Lee, M-Y. A Pillar and Perfusion Plate Platform for Robust Human Organoid Culture and Analysis. Advanced healthcare materials. 2024; 13(21):e2302502.

Sanchez, JG; Rankin, S; Paul, E; Mccauley, HA; Kechele, DO; Enriquez, JR; Jones, N-H; Greeley, SAW; Letourneau-Frieberg, L; Zorn, AM; Krishnamurthy, M; Wells, JM. RFX6 regulates human intestinal patterning and function upstream of PDX1. Development. 2024; 151(9).

Gilbert, PM; Hofmann, S; Ng, H-H; Vankelecom, H; Wells, JM. Organoids in endocrine and metabolic research: current and emerging applications. Nature Reviews Endocrinology. 2024; 20(4):195-201.

Rivron, NC; Martinez-Arias, A; Sermon, K; Mummery, C; Schöler, HR; Wells, J; Nichols, J; Hadjantonakis, A-K; Lancaster, MA; Moris, N; Fu, J; Sturmey, RG; Niakan, K; Rossant, J; Kato, K. Changing the public perception of human embryology. Nature Cell Biology. 2023; 25(12):1717-1719.

Múnera, JO; Kechele, DO; Bouffi, C; Qu, N; Jing, R; Maity, P; Enriquez, JR; Han, L; Campbell, I; Mahe, MM; Hu, Y-C; Takebe, T; Helmrath, MA; Wells, JM. Development of functional resident macrophages in human pluripotent stem cell-derived colonic organoids and human fetal colon. Cell Stem Cell. 2023; 30(11):1434-1451.e9.

Bouffi, C; Wikenheiser-Brokamp, KA; Chaturvedi, P; Sundaram, N; Goddard, GR; Wunderlich, M; Brown, NE; Staab, JF; Latanich, R; Zachos, NC; Mulloy, JC; Zorn, AM; Wells, JM; Helmrath, MA. In vivo development of immune tissue in human intestinal organoids transplanted into humanized mice. Nature Biotechnology. 2023; 41(6):824-831.

Marek-Yagel, D; Stenke, E; Pode-Shakked, B; Dunne, C; Crushell, E; Bryce-Smith, A; Mcdermott, M; O'sullivan, MJ; Veber, A; Krishnamurthy, M; Wells, JM; Anikster, Y; Bourke, B. Nonsense mutation in the novel PERCC1 gene as a genetic cause of congenital diarrhea and enteropathy. Human Genetics. 2023; 142(5):691-696.

Singh, A; Poling, HM; Chaturvedi, P; Thorner, K; Sundaram, N; Kechele, DO; Childs, CJ; Mccauley, HA; Fisher, GW; Brown, NE; Spence, JR; Wells, JM; Helmrath, MA. Transplanted human intestinal organoids: a resource for modeling human intestinal development. Development. 2023; 150(9).

Mccauley, HA; Riedman, AM; Enriquez, JR; Zhang, X; Watanabe-Chailland, M; Sanchez, JG; Kechele, DO; Paul, EF; Riley, K; Burger, C; Lang, RA; Wells, JM. Enteroendocrine Cells Protect the Stem Cell Niche by Regulating Crypt Metabolism in Response to Nutrients. Cellular and Molecular Gastroenterology and Hepatology (CMGH). 2023; 15(6):1293-1310.

Rosselot, AE; Park, M; Kim, M; Matsu-Ura, T; Wu, G; Flores, DE; Subramanian, KR; Lee, S; Sundaram, N; Broda, TR; Wells, JM; Hogenesch, JB; Moore, SR; Hong, CI. Ontogeny and function of the circadian clock in intestinal organoids. EMBO Journal. 2022; 41(2):e106973.

Cable, J; Lutolf, MP; Fu, J; Park, SE; Apostolou, A; Chen, S; Song, CJ; Spence, JR; Liberali, P; Lancaster, M; Gouti, M; Hombrink, P; Quadrato, G; Urenda, J-P. Organoids as tools for fundamental discovery and translation-a Keystone Symposia report. Annals of the New York Academy of Sciences. 2022; 1518(1):196-208.

Katz, AE; Yang, M-L; Levin, MG; Tcheandjieu, C; Mathis, M; Hunker, K; Blackburn, S; Eliason, JL; Coleman, DM; Fendrikova-Mahlay, N; Li, JZ; Damrauer, SM; Stanley, JC; Ganesh, SK. Fibromuscular Dysplasia and Abdominal Aortic Aneurysms Are Dimorphic Sex-Specific Diseases With Shared Complex Genetic Architecture. Circulation. Genomic and precision medicine. 2022; 15(6):e003496.

Enriquez, JR; Mccauley, HA; Zhang, KX; Sanchez, JG; Kalin, GT; Lang, RA; Wells, JM. A dietary change to a high-fat diet initiates a rapid adaptation of the intestine. Cell reports. 2022; 41(7):111641.

Wells, JM; Guo, Z. In preprints: humans, the new model organism. Development. 2022; 149(22).

Kishimoto, K; Iwasawa, K; Sorel, A; Ferran-Heredia, C; Han, L; Morimoto, M; Wells, JM; Takebe, T; Zorn, AM. Directed differentiation of human pluripotent stem cells into diverse organ-specific mesenchyme of the digestive and respiratory systems. Nature Protocols. 2022; 17(11):2699-2719.

Wells, JM. A renaissance for developmental biology driven by new in vitro platforms. Development. 2022; 149(20).

Krishnamurthy, M; Kechele, DO; Broda, T; Zhang, X; Enriquez, JR; Mccauley, HA; Sanchez, JG; Mccracken, K; Palermo, J; Bernieh, A; Neef, HC; Heider, A; Dauber, A; Wells, JM. Using Human Induced Pluripotent Stem Cell-Derived Organoids to Identify New Pathologies in Patients With PDX1 Mutations. Gastroenterology. 2022; 163(4):1053-1063.e7.

Pitstick, AL; Poling, HM; Sundaram, N; Lewis, PL; Kechele, DO; Sanchez, JG; Scott, MA; Broda, TR; Helmrath, MA; Wells, JM; Mayhew, CN. Aggregation of cryopreserved mid-hindgut endoderm for more reliable and reproducible hPSC-derived small intestinal organoid generation. Stem Cell Reports. 2022; 17(8):1889-1902.

Jurickova, I; Bonkowski, E; Angerman, E; Novak, E; Huron, A; Akers, G; Iwasawa, K; Braun, T; Hadar, R; Hooker, M; Takebe, T; Mollen, KP; Haberman, Y; Denson, LA. Eicosatetraynoic Acid and Butyrate Regulate Human Intestinal Organoid Mitochondrial and Extracellular Matrix Pathways Implicated in Crohn's Disease Strictures. Inflammatory Bowel Diseases. 2022; 28(7):988-1003.

Sanchez, JG; Enriquez, JR; Wells, JM. Enteroendocrine cell differentiation and function in the intestine. Current Opinion in Endocrinology, Diabetes and Obesity. 2022; 29(2):169-176.

Eicher, AK; Kechele, DO; Sundaram, N; Berns, HM; Poling, HM; Haines, LE; Sanchez, JG; Kishimoto, K; Krishnamurthy, M; Han, L; Zorn, AM; Helmrath, MA; Wells, JM. Functional human gastrointestinal organoids can be engineered from three primary germ layers derived separately from pluripotent stem cells. Cell Stem Cell. 2022; 29(1):36-51.e6.

Edwards, NA; Shacham-Silverberg, V; Weitz, L; Kingma, PS; Shen, Y; Wells, JM; Chung, WK; Zorn, AM. Developmental basis of trachea-esophageal birth defects. Developmental Biology. 2021; 477:85-97.

Lewis, PL; Wells, JM. Engineering-inspired approaches to study β-cell function and diabetes. Stem Cells. 2021; 39(5):522-535.

Trisno, SL; Higano, NS; Kechele, D; Nasr, T; Chung, W; Zorn, AM; Woods, JC; Wells, JM; Kingma, PS. Case Report: Esophageal Bronchus in a Neonate, With Image, Histological, and Molecular Analysis. Frontiers in Pediatrics. 2021; 9:707822.

Lee, KKP; Matsu-Ura, T; Rosselot, AE; Broda, TR; Wells, JM; Hong, CI. An integrated microfluidic bubble pocket for long-term perfused three-dimensional intestine-on-a-chip model. Biomicrofluidics. 2021; 15(1):014110.

Nasr, T; Holderbaum, AM; Chaturvedi, P; Agarwal, K; Kinney, JL; Daniels, K; Trisno, SL; Ustiyan, V; Shannon, JM; Wells, JM; Sinner, D; Kalinichenko, VV; Zorn, AM. Disruption of a hedgehog-foxf1-rspo2 signaling axis leads to tracheomalacia and a loss of sox9+ tracheal chondrocytes. Disease Models & Mechanisms. 2020; 14(2).

Kasendra, M; Wells, JM. A Window into Your Gut: Biologically Inspired Engineering of Mini-gut Tubes In Vitro. Developmental Cell. 2020; 55(5):522-524.

Mccauley, HA; Matthis, AL; Enriquez, JR; Nichol, JT; Sanchez, JG; Stone, WJ; Sundaram, N; Helmrath, MA; Montrose, MH; Aihara, E; Wells, JM. Enteroendocrine cells couple nutrient sensing to nutrient absorption by regulating ion transport. Nature Communications. 2020; 11(1):4791.

Singh, A; Poling, HM; Spence, JR; Wells, JM; Helmrath, MA. Gastrointestinal organoids: a next-generation tool for modeling human development. American Journal of Physiology - Gastrointestinal and Liver Physiology. 2020; 319(3):G375-G381.

Han, L; Chaturvedi, P; Kishimoto, K; Koike, H; Nasr, T; Iwasawa, K; Giesbrecht, K; Witcher, PC; Eicher, A; Haines, L; Morimoto, M; Wells, JM; Takebe, T; Zorn, AM. Single cell transcriptomics identifies a signaling network coordinating endoderm and mesoderm diversification during foregut organogenesis. Nature Communications. 2020; 11(1):4158.

Nowrouzi, A; Sertorio, MG; Akbarpour, M; Knoll, M; Krunic, D; Kuhar, M; Schwager, C; Brons, S; Debus, J; Wells, SI; Wells, JM; Abdollahi, A. Personalized Assessment of Normal Tissue Radiosensitivity via Transcriptome Response to Photon, Proton and Carbon Irradiation in Patient-Derived Human Intestinal Organoids. Cancers. 2020; 12(2).

Pradhan, S; Karve, SS; Weiss, AA; Hawkins, J; Poling, HM; Helmrath, MA; Wells, JM; Mccauley, HA. Tissue Responses to Shiga Toxin in Human Intestinal Organoids. Cellular and Molecular Gastroenterology and Hepatology (CMGH). 2020; 10(1):171-190.

Shacham-Silverberg, V; Wells, JM. Generation of esophageal organoids and organotypic raft cultures from human pluripotent stem cells. 2020:1-22.

Singh, A; Poling, HM; Sundaram, N; Brown, N; Wells, JM; Helmrath, MA. Evaluation of transplantation sites for human intestinal organoids. PLoS ONE. 2020; 15(8):e0237885.