The research projects in Dr. Helmrath's lab rely on funding from the following sources:
Limited Competition for the Continuation of Teen-LABS (Adolescent Longitudinal Assessment of Bariatric Surgery) Clinical Centers (Collaborative U01)
"This funding will support a third cycle of the Teen-LABS consortium to conduct long term (10 year) follow up of the adolescent bariatric cohort. This cycle of the study will address new scientific aims relevant to late effects (risks and benefits) of bariatric surgery performed in youth."
UM1DK072493 Inge (PI), NIH/NIDDK
Multi-institutional trial of non-operative management of uncomplicated pediatric appendicitis
“This study is a mulit-institutional trial to determine the effectiveness of non-operative management as an alternative first line therapy for children with uncomplicated appendicitis.”
Patient Centered Outcomes Minneci (PI), Research Institute (PCORI)
The Role of Hedgehog Signaling in gastric tissue repair and regeneration
"Dr. Helmrath is responsible for the sleeve gastrectomies and collection of human fundic tissue. He will also assist Dr. Zavros with the development and culture of the human fundic organoids. Dr. Helmrath will also share the responsibility with Dr. Zavros with regards to the interpretation of data, experimental design, and preparation of manuscripts."
R01DK083402 Zavros (PI), NIH/NIDDK
Establishment of in vitro and in vivo models of human gastrointestinal organoids with a functional ENS
"In this application we propose to incorporate an ENS into other regional organoids, including recently established gastric and colonic organoids, and to generate live-imaging reporter systems to study ENS function in vitro and in vivo."
U18EB021780 Wells/Helmrath (PI), NIH/NIBIB
Personalizing Cystic Fibrosis Research Translation
"Clinically, his role as the director of the Intestinal Rehabilitation Program at CCHMC provides access to all patients with intestinal failure/diseases. The development of a tissue bank from this population of patients provides a valuable resource to study human ISC. He will provide surgical expertise and oversee all in vivo models in this study. He personally performs many of the surgeries involving anastomosis to intestine."
CLANCY15R0 Clancy (PI), Cystic Fibrosis Foundation Role: Co-Investigator
LPA2 receptor-containing complexes in regulating secretory diarrhea
"This application proposes to develop a physiologically relevant human intestinal stem cells (enteroids) to study CFTR-dependent fluid secretion and develop assays to demonstrate and identify LPA2 receptor specific small molecule agonists. LPA2 dependent inhibition of CFTR requires adenylate cyclase 6 (AC6) suggesting that AC6 is part of the CFTR-NHERF2-LPA2 complex. Dr. Helmrath’s role as the director of the Intestinal Rehabilitation Program at CCHMC provides access to all patients with intestinal failure/diseases. The development of a tissue bank from this population of patients provides a valuable resource to study human enteroids. Dr. Helmrath will help with the isolation of human enteroids."
R01DK093045 Naren (PI), NIDDK
Intestinal Organoids as a model system for studying enteric disease
"My role in this project is to provide intestinal organoids from human subjects including; newborns, adolescents, and adults over 65 years of age."
U19AI116491-01 Weiss (PI), NIH/NIAID
Investigation of Regional Identity in Human Intestinal Stem Cells
"The proposed work utilizes cutting edge technology to characterize the network of molecular determinants of regionalized intestinal stem cells. This work will lead to a deeper understanding of regional influence within intestinal stem cell populations that may contribute to physiological and disease specific difference commonly seen between the proximal and distal intestine."
U01DK103117 Helmrath (PI), NIH/NIDDK
Outcome of NASH in Adolescents after Bariatric Surgery vs. Lifestyle Intervention
“The overall objective of this project is to assess the effectiveness of bariatric or weight loss surgery (WLS) as a treatment for nonalcoholic steatohepatitis (NASH) in severely obese adolescents, compared with a comprehensive lifestyle intervention (CLI).”
R01 DK100429 Xanthakos (PI), NIH/NIDDK
Role: Co- Investigator
Adolescent Bariatric Surgery: Weight and Psychosocial Risk in Young Adulthood
"Obesity, mental health, the engagement in HIV/sexual-risk behaviors, alcohol/tobacco/drug use, as well as suicidal behaviors are significant public health priorities for adolescents and young adults. Bariatric or weight loss surgery (WLS) has become a viable treatment option for adolescents whose obesity has progressed to extreme levels (BMI>40 kg/m)."
R01DK080020 Zeller (PI), NIH/NIDDK
Role: Co- Investigator
Single Cell/RNA-Seq dissection of Human iPS cell development into intestine
"The aims of this grant use a combination of new technologies, allowing the generation of human intestine from iPS cells, and allowing the RNA-Seq gene expression analysis of single cells, to create an atlas of the gene expression programs that drive the differentiation of the distinct intestinal lineages."
R01DK098350 Potter (PI), NIH/NIDDK
Patient Specific Enteroids from Small Intestine and Colon
"Clinically, his role as the director of the Intestinal Rehabilitation Program at CCHMC provides access to all patients with intestinal failure/diseases. The development of a tissue bank from this population of patients provides a valuable resource to study human ISC. Dr. Helmrath will collect patient samples and oversee this Core at CCHMC."
Naren14XXO Naren (PI). Cystic Fibrosis Foundation
Developmental Mechanisms of Trachea-Esophageal Birth Defects
“Helmrath has established and directs the human intestinal repository and culture facility. Dr. Helmrath and his team will perform surgical transplants of organoids into mice.”
P01 HD093363-01 Zorn (PI), NIH
Human Enteroids, Colonoids, and iPSC derived HIO's to study CFTR-related Disorders
“Clinically, his role as the director of the Intestinal Rehabilitation Program at CCHMC provides access to all patients with intestinal failure/diseases. The development of a tissue bank from this population of patients provides a valuable resource to study human enteroids. Dr. Helmrath will help with the isolation of human enteroids and assist in HIOs generation in kidney capsule.”
R01 DK112321-01A1 Naren (PI), NIH