Left to Right: Joe Shiley, Phoebe Franz, Chris Mayhew, Amy Pitstick
The Pluripotent Stem Cell Facility originated in 2008. Our services and training have expanded significantly through the years, but our mission remains constant.
The PSCF is dedicated to providing high quality, well characterized, and reliably archived human embryonic stem cells (hESCs) and induced pluripotent stem cells (iPSCs) for distribution to researchers. Additionally, the facility will provide investigators with expert training in the protocols and techniques for proper handling and manipulation of hPSCs. Our overall mission is to facilitate and support all aspects of pluripotent stem cell experimentation to contribute to advancing stem cell therapies for human disease.
• Establish a bank of well characterized, quality-controlled hESCs and iPSCs
• Provide reagents and expertise for the generation of human iPSCs
• Provide reagents and expertise for gene-editing hPSCs
• Make these methods and materials available to Cincinnati researchers
• Provide training in the culture and manipulation of hESCs and iPSCs
• Support the utilization and development of protocols for the directed hESC/iPSC differentiation
• Assist investigators with experimental design as well as data analysis and interpretation
• Provide support for manuscript and grant proposal development
Click here for a description of our service and training offerings.
Advantages of Using the Stem Cell Facility
The facility has all the necessary expertise, equipment, reagents and regulatory approvals to perform most experiments in-house. Performing your experiments in our facility has several advantages:
• Significant savings in time and cost versus establishing procedures and approvals in investigator labs*
• Assistance with experimental design and data interpretation
• Access to hands-on training
• Access to quality-controlled reagents
• The ability to perform approved experiments using our ESCRO approval
*Investigators can purchase and use their own hESCs independently of the PSCF but are then responsible for obtaining ESCRO approval, appropriate IACUC and IBC permissions, as well as permission to use the cells from the cell line originator.
James Wells PhD (Director)
Chris Mayhew PhD (Co-Director)
Amy Pitstick MS (Research Assistant)
Phoebe Franz MS (Research Assistant)
Joseph Shiley MS (Research Assistant)
Cincinnati Children's Hospital Medical Center
Division of Developmental Biology
Location R, Room 3469
Cincinnati, OH 45229
Phone [office]: 513-636-9254
Phone [lab]: 513-636-3744