The Pluripotent Stem Cell Facility originated in 2008. Our services and training have expanded significantly through the years, but our mission remains constant.

Our Mission

The PSCF is dedicated to providing high quality, well characterized, and reliably archived human embryonic and induced pluripotent stem cells for distribution to researchers. Additionally, the facility will provide investigators with expert training in the protocols and techniques for proper handling and manipulation of human embryonic stem cells. Our overall mission is to facilitate all aspects of pluripotent stem cell experimentation to contribute to advancing stem cell therapies for human disease.

Our Objectives

Establish a bank of well characterized, quality controlled human embryonic stem cells (hESC) and induced pluripotent (iPSC) stem cells

  • Provide expertise in the generation of iPSCs
  • Provide access to custom iPSC genome editing services
  • Provide training in the culture and manipulation of hESCs and iPSCs
  • Utilize and develop protocols for directed hESC/iPSC differentiation
  • Assist investigators in experimental design as well as data analysis and interpretation
  • Provide support for manuscript and grant proposal development

Advantages of Using the Pluripotent Stem Cell Facility

The facility has all the necessary expertise, equipment, reagents and regulatory approvals to perform most experiments in-house. Performing your experiments in our facility has several advantages:

  • Significant savings in time and cost versus establishing procedures and approvals in investigator labs
  • Assistance with experimental design and data interpretation
  • Access to hands-on training
  • Access to quality controlled reagents
  • The ability to perform approved experiments using our ESCRO approval

The PSCF offers most services to non-Cincinnati Children's / UC investigators. Please contact us for details.

Contact Us

Chris Mayhew, PhD (Director)
Amy Pitstick, MS (PSCF manager)
Joseph Shiley, MS (hPSC gene editing service manager)
Kyle MacKenzie (Research Assistant)
Ishe Mazani (Research Assistant)
Alex Choi (Research Assistant)

Cincinnati Children's Hospital Medical Center
Division of Developmental Biology
Location R, Room 3-005
Cincinnati, OH 45229
Phone [office]: 513-636-9254
Phone [lab]: 513-636-3744