Takebe Lab

Takebe Research Lab

Modeling Complex Human Development Towards Therapy

On the basis of "reverse reduction-ism based approach," the Takebe Laboratory investigates:

The deductive development of a complex human organoid model
The multidisciplinary dissection of self-driven mechanisms of organogenesis
The technology prototyping towards drug discovery/transplant applications

Through these efforts, with a major focus on foregut- and midgut-derived systems, our lab aims to contribute to human health by establishing new therapies especially for treating pediatric intractable disease.

Also, Dr Takebe holds a joint appointment at the Department of Regenerative Medicine, Yokohama City University in Japan, where he directs a translational program of human induced pluripotent stem cell (iPSC) based liver bud based approach for congenital liver disorders by developing a cGMP manufacturing platform.

Stem cell biology and organoid biology: Think Radically graphic.

Publications

2020

Sakabe, K; Takebe, T; Asai, A. Organoid Medicine in Hepatology. Clinical Liver Disease. 2020; 15:3-8.

2019

Fang, H; Yao, S; Chen, Q; Liu, C; Cai, Y; Geng, S; Bai, Y; Tian, Z; Zacharias, AL; Takebe, T; et al. De Novo-Designed Near-Infrared Nanoaggregates for Super-Resolution Monitoring of Lysosomes in Cells, in Whole Organoids, and in Vivo. ACS Nano. 2019; 13:14426-14436.

Liu, T; Zhou, L; Yang, K; Iwasawa, K; Kadekaro, AL; Takebe, T; Andl, T; Zhang, Y. The β-catenin/YAP signaling axis is a key regulator of melanoma-associated fibroblasts. Signal Transduction and Targeted Therapy. 2019; 4.

Koike, H; Iwasawa, K; Ouchi, R; Maezawa, M; Giesbrecht, K; Saiki, N; Ferguson, A; Kimura, M; Thompson, WL; Wells, JM; et al. Modelling human hepato-biliary-pancreatic organogenesis from the foregut–midgut boundary. Nature. 2019; 574:112-116.

Ouchi, R; Togo, S; Kimura, M; Shinozawa, T; Koido, M; Koike, H; Thompson, W; Karns, RA; Mayhew, CN; McGrath, PS; et al. Modeling Steatohepatitis in Humans with Pluripotent Stem Cell-Derived Organoids. Cell Metabolism. 2019; 30:374-384.e6.

Takebe, T; Wells, JM. Organoids by design. Science. 2019; 364:956-959.

Iizuka, S; Takebe, T; Nishii, S; Kodaka, A. (2019) Approach for Communication Design for Motivation to Health Behavior. Springer International Publishing. 11569 LNCS:425-436.

2018

Matsuzaki, T; Matsumoto, S; Kasai, T; Yoshizawa, E; Okamoto, S; Yoshikawa, HY; Taniguchi, H; Takebe, T. Defining Lineage-Specific Membrane Fluidity Signatures that Regulate Adhesion Kinetics. Stem Cell Reports. 2018; 11:852-860.

Lewis, K; Takebe, T. Tumoroid a la carte: Path for personalization. Hepatology. 2018; 68:1189-1191.

Nie, Y; Zheng, Y; Miyakawa, K; Murata, S; Zhang, R; Sekine, K; Ueno, Y; Takebe, T; Wakita, T; Ryo, A; et al. Recapitulation of hepatitis B virus-host interactions in liver organoids from human induced pluripotent stem cells. EBioMedicine. 2018; 35:114-123.

Ayabe, H; Anada, T; Kamoya, T; Sato, T; Kimura, M; Yoshizawa, E; Kikuchi, S; Ueno, Y; Sekine, K; Camp, JG; et al. Optimal Hypoxia Regulates Human iPSC-Derived Liver Bud Differentiation through Intercellular TGFB Signaling. Stem Cell Reports. 2018; 11:306-316.

Kimura, M; Azuma, M; Zhang, R; Thompson, W; Mayhew, CN; Takebe, T. Digitalized Human Organoid for Wireless Phenotyping. iScience. 2018; 4:294-301.

Rao, MS; Pei, Y; Garcia, TY; Chew, S; Kasai, T; Hisai, T; Taniguchi, H; Takebe, T; Lamba, DA; Zeng, X. Illustrating the potency of current Good Manufacturing Practice-compliant induced pluripotent stem cell lines as a source of multiple cell lineages using standardized protocols. Cytotherapy (Informa). 2018; 20:861-872.

Takebe, T; Wells, JM; Helmrath, MA; Zorn, AM. Organoid Center Strategies for Accelerating Clinical Translation. Cell Stem Cell. 2018; 22:806-809.

Takahashi, Y; Sekine, K; Kin, T; Takebe, T; Taniguchi, H. Self-Condensation Culture Enables Vascularization of Tissue Fragments for Efficient Therapeutic Transplantation. Cell Reports. 2018; 23:1620-1629.

Takahashi, Y; Takebe, T; Taniguchi, H. Methods for Generating Vascularized Islet-Like Organoids Via Self-Condensation. Current Protocols in Stem Cell Biology. 2018; 45:e49-e49.

Zhang, R; Koido, M; Tadokoro, T; Ouchi, R; Matsuno, T; Ueno, Y; Sekine, K; Takebe, T; Taniguchi, H. Human iPSC-Derived Posterior Gut Progenitors Are Expandable and Capable of Forming Gut and Liver Organoids. Stem Cell Reports. 2018; 10:780-793.

2017

Takebe, T; Sekine, K; Kimura, M; Yoshizawa, E; Ayano, S; Koido, M; Funayama, S; Nakanishi, N; Hisai, T; Kobayashi, T; et al. Massive and Reproducible Production of Liver Buds Entirely from Human Pluripotent Stem Cells. Cell Reports. 2017; 21:2661-2670.

Takebe, T; Zhang, B; Radisic, M. Synergistic Engineering: Organoids Meet Organs-on-a-Chip. Cell Stem Cell. 2017; 21:297-300.

Takebe, T; Sekine, K; Camp, G; Treutlein, B. Human Liver Bud Organoid Models Development. Cell Systems. 2017; 5:2-2.

Camp, JG; Sekine, K; Gerber, T; Loeffler-Wirth, H; Binder, H; Gac, M; Kanton, S; Kageyama, J; Damm, G; Seehofer, D; et al. Multilineage communication regulates human liver bud development from pluripotency. Nature. 2017; 546:533-538.

Asai, A; Aihara, E; Watson, C; Mourya, R; Mizuochi, T; Shivakumar, P; Phelan, K; Mayhew, C; Helmrath, M; Takebe, T; et al. Paracrine signals regulate human liver organoid maturation from induced pluripotent stem cells. Development (Cambridge). 2017; 144:1056-1064.

Koike, H; Zhang, R; Ueno, Y; Sekine, K; Zheng, Y; Takebe, T; Taniguchi, H. Nutritional modulation of mouse and human liver bud growth through a branched-chain amino acid metabolism. Development (Cambridge). 2017; 144:1018-1024.

Ito, K; Sakuma, S; Kimura, M; Takebe, T; Kaneko, M; Arai, F; IEEE, . (2017) Mechanical characterization system using on-chip probe with wide range actuation. IEEE.

Sekine, K; Takebe, T; Taniguchi, H. Liver regeneration using cultured liver bud. Methods in molecular biology (Clifton, N.J.). 2017; 1597:207-216.

Sekine, K; Takebe, T; Taniguchi, H. Liver regeneration using cultured liver bud. Organ Regeneration Based on Developmental Biology. : Springer Singapore; Springer Singapore; 2017.

2016

Shinozawa, T; Yoshikawa, HY; Takebe, T. Reverse engineering liver buds through self-driven condensation and organization towards medical application. Developmental Biology. 2016; 420:221-229.

Ito, K; Sakuma, S; Kimura, M; Takebe, T; Kaneko, M; Arai, F. Temporal Transition of Mechanical Characteristics of HUVEC/MSC Spheroids Using a Microfluidic Chip with Force Sensor Probes. Micromachines. 2016; 7:221-221.

Koike, H; Rashid, T; Takebe, T. Growing Mini-Organs from Stem Cells. In: Meyers RA, Ed. Reviews in Cell Biology and Molecular Medicine. Weinheim, Germany: Wiley-VCH Verlag GmbH & Co. KGaA; 2016.

Koike, H; Takebe, T. Generating Mini-Organs in Culture. Current Pathobiology Reports. 2016; 4:59-68.

Kagimoto, S; Takebe, T; KobayashiA, S; Yabukig, Y; Hori, A; Hirotomi, K; Mikami, T; Uemura, T; Maegawa, J; Taniguchi, H. Autotransplantation of Monkey Ear Perichondrium-Derived Progenitor Cells for Cartilage Reconstruction. Cell Transplantation. 2016; 25:951-962.

Takahashi, Y; Takebe, T; Taniguchi, H. Engineering pancreatic tissues from stem cells towards therapy. Regenerative Therapy. 2016; 3:15-23.

Ito, K; Sakuma, S; Kimura, M; Takebe, T; Kaneko, M; Arai, F; IEEE, . (2016) STIFFNESS-INDEX MAP BASED ON SINGLE CELL-SPHEROID ANALYSIS USING ROBOT INTEGRATED MICROFLUIDIC CHIIP. IEEE. 2016-February:157-160.

JSPS PRIZE Recipient

Dr. Takanori Takebe was selected as one of six young Japanese researchers to receive the JSPS PRIZE. The Japan Society for the Promotion of Science (JSPS) offers this annual award to recognize and support young researchers with superlative research abilities in early stages of their careers. Recipients have the potential to become world leaders in their fields. Dr. Takebe will receive the award from the Imperial Prince of Japan in February 2019. Learn more about PRIZE

Contact Us

Takanori Takebe, MD
Assistant Professor, UC Department of Pediatrics

Mailing Address
Division of Gastroenterology, Hepatology and Nutrition & Division of Developmental Biology
3333 Burnet Ave.
MLC 2010
Cincinnati, OH 45229-3039

Lab: Location R, Room 3543

Office: Location S, Suite S3.622

Phone: 513-803-7807
Fax: 513-636-5581
Email: Takanori.Takebe@cchmc.org

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