Millay Lab

Publications

Hindi, SM; Petrany, MJ; Greenfeld, E; Focke, LC; Cramer, AA W; Whitt, MA; Khairallah, RJ; Ward, CW; Chamberlain, JS; Podbilewicz, B; Prasad, V; Millay, DP. Enveloped viruses pseudotyped with mammalian myogenic cell fusogens target skeletal muscle for gene delivery. Cell. 2023; 186:2062-2077.e17.

Gamage, DG; Melikov, K; Munoz-Tello, P; Wherley, TJ; Focke, LC; Leikina, E; Huffman, E; Diao, J; Kojetin, DJ; Prasad, V; Chernomordik, LV; Millay, DP. Phosphatidylserine orchestrates Myomerger membrane insertions to drive myoblast fusion. Proceedings of the National Academy of Sciences of USA. 2022; 119:e2202490119.

Eigler, T; Zarfati, G; Amzallag, E; Sinha, S; Segev, N; Zabary, Y; Zaritsky, A; Shakked, A; Umansky, KB; Schejter, ED; Millay, DP; Tzahor, E; Avinoam, O. ERK1/2 inhibition promotes robust myotube growth via CaMKII activation resulting in myoblast-to-myotube fusion. Developmental Cell. 2021; 56:3349-3363.e6.

Prasad, V; Millay, DP. Skeletal muscle fibers count on nuclear numbers for growth. Seminars in Cell and Developmental Biology. 2021; 119:3-10.

Petrany, MJ; Swoboda, CO; Sun, C; Chetal, K; Chen, X; Weirauch, MT; Salomonis, N; Millay, DP. Single-nucleus RNA-seq identifies transcriptional heterogeneity in multinucleated skeletal myofibers. Nature Communications. 2020; 11:6374.

Cramer, AA W; Prasad, V; Eftestøl, E; Song, T; Hansson, KA; Dugdale, HF; Sadayappan, S; Ochala, J; Gundersen, K; Millay, DP. Nuclear numbers in syncytial muscle fibers promote size but limit the development of larger myonuclear domains. Nature Communications. 2020; 11:6287.

Petrany, MJ; Song, T; Sadayappan, S; Millay, DP. Myocyte-derived Myomaker expression is required for regenerative fusion but exacerbates membrane instability in dystrophic myofibers. JCI insight. 2020; 5:136095.

Goh, Q; Song, T; Petrany, MJ; Cramer, AA W; Sun, C; Sadayappan, S; Lee, S; Millay, DP. Myonuclear accretion is a determinant of exercise-induced remodeling in skeletal muscle. eLife. 2019; 8:e44876.

Quinn, ME; Goh, Q; Kurosaka, M; Gamage, DG; Petrany, MJ; Prasad, V; Millay, DP. Myomerger induces fusion of non-fusogenic cells and is required for skeletal muscle development. Nature Communications. 2017; 8:15665.

Sun, C; Swoboda, CO; Morales, FM; Calvo, C; Petrany, MJ; Parameswaran, S; VonHandorf, A; Weirauch, MT; Lepper, C; Millay, DP. Lineage tracing of nuclei in skeletal myofibers uncovers distinct transcripts and interplay between myonuclear populations. Nature Communications. 2024; 15:9372.

Franco-Romero, A; Morbidoni, V; Milan, G; Sartori, R; Wulff, J; Romanello, V; Armani, A; Salviati, L; Conte, M; Salvioli, S; Millay, DP; Tooze, SA; Trevisson, E; Sandri, M. C16ORF70/MYTHO promotes healthy aging in C.elegans and prevents cellular senescence in mammals. The Journal of Clinical Investigation. 2024; 134:e165814.

Prabakaran, AD; McFarland, K; Miz, K; Durumutla, HB; Piczer, K; El Abdellaoui Soussi, F; Latimer, H; Werbrich, C; Chung, HJ; Blair, NS; Millay, DP; Morris, AJ; Prideaux, B; Finck, BN; Quattrocelli, M. Intermittent glucocorticoid treatment improves muscle metabolism via the PGC1α/Lipin1 axis in an aging-related sarcopenia model. The Journal of Clinical Investigation. 2024; 134:e177427.

Grimes, KM; Maillet, M; Swoboda, CO; Bowers, SL K; Millay, DP; Molkentin, JD. MEK1-ERK1/2 signaling regulates the cardiomyocyte non-sarcomeric actin cytoskeletal network. American Journal of Physiology: Heart and Circulatory Physiology. 2024; 326:H180-H189.

Wherley, TJ; Thomas, S; Millay, DP; Saunders, T; Roy, S. Molecular regulation of myocyte fusion. Current Topics in Developmental Biology. 2024; 158:53-82.

Witcher, PC; Sun, C; Millay, DP. Expression of Myomaker and Myomerger in myofibers causes muscle pathology. Skeletal Muscle. 2023; 13:8.

Grimes, KM; Bowers, SL; Swoboda, CO; Millay, DP; Molkentin, JD. Abstract P2023: Mitogen-activated Protein Kinase-directed Modulation Of The Non-sarcomeric Actin Cytoskeleton In Cardiomyocytes. Circulation Research. 2023; 133.

O'Donnell, A; Gonzalez, BA; Mukherjee, S; Wilson, R; Alfieri, CM; Swoboda, CO; Millay, DP; Zorn, AM; Yutzey, KE. Localized Prox1 Regulates Aortic Valve Endothelial Cell Diversity and Extracellular Matrix Stratification in Mice. Arteriosclerosis, Thrombosis, and Vascular Biology. 2023; 43:1478-1493.

Hindi, SM; Petrany, MJ; Greenfeld, E; Focke, LC; Cramer, AA W; Whitt, MA; Khairallah, RJ; Ward, CW; Chamberlain, JS; Podbilewicz, B; Prasad, V; Millay, DP. Enveloped viruses pseudotyped with mammalian myogenic cell fusogens target skeletal muscle for gene delivery. Cell. 2023; 186:2062-2077.e17.

Gamage, DG; Melikov, K; Munoz-Tello, P; Wherley, TJ; Focke, LC; Leikina, E; Huffman, E; Diao, J; Kojetin, DJ; Prasad, V; Chernomordik, LV; Millay, DP. Phosphatidylserine orchestrates Myomerger membrane insertions to drive myoblast fusion. Proceedings of the National Academy of Sciences of USA. 2022; 119:e2202490119.

Hindi, SM; Millay, DP. All for One and One for All: Regenerating Skeletal Muscle. Cold Spring Harbor perspectives in biology. 2022; 14:a040824.

Millay, DP. Regulation of the myoblast fusion reaction for muscle development, regeneration, and adaptations. Experimental Cell Research. 2022; 415:113134.

Eigler, T; Zarfati, G; Amzallag, E; Sinha, S; Segev, N; Zabary, Y; Zaritsky, A; Shakked, A; Umansky, KB; Schejter, ED; Millay, DP; Tzahor, E; Avinoam, O. ERK1/2 inhibition promotes robust myotube growth via CaMKII activation resulting in myoblast-to-myotube fusion. Developmental Cell. 2021; 56:3349-3363.e6.

Girardi, F; Taleb, A; Ebrahimi, M; Datye, A; Gamage, DG; Peccate, C; Giordani, L; Millay, DP; Gilbert, PM; Cadot, B; Le Grand, F. TGFβ signaling curbs cell fusion and muscle regeneration. Nature Communications. 2021; 12:750.

Yaseen, W; Kraft-Sheleg, O; Zaffryar-Eilot, S; Melamed, S; Sun, C; Millay, DP; Hasson, P. Fibroblast fusion to the muscle fiber regulates myotendinous junction formation. Nature Communications. 2021; 12:3852.

Golani, G; Leikina, E; Melikov, K; Whitlock, JM; Gamage, DG; Luoma-Overstreet, G; Millay, DP; Kozlov, MM; Chernomordik, LV. Myomerger promotes fusion pore by elastic coupling between proximal membrane leaflets and hemifusion diaphragm. Nature Communications. 2021; 12:495.

Bengtsen, M; Winje, IM; Eftestøl, E; Landskron, J; Sun, C; Nygård, K; Domanska, D; Millay, DP; Meza-Zepeda, LA; Gundersen, K. Comparing the epigenetic landscape in myonuclei purified with a PCM1 antibody from a fast/glycolytic and a slow/oxidative muscle. Editor, Blewitt ME. PLoS Genetics. 2021; 17:e1009907.

Prasad, V; Millay, DP. Skeletal muscle fibers count on nuclear numbers for growth. Seminars in Cell and Developmental Biology. 2021; 119:3-10.

Milewski, D; Shukla, S; Gryder, BE; Pradhan, A; Donovan, J; Sudha, P; Vallabh, S; Pyros, A; Xu, Y; Barski, A; Millay, DP; Khan, J; Kalinichenko, VV; Kalin, TV. FOXF1 is required for the oncogenic properties of PAX3-FOXO1 in rhabdomyosarcoma. Oncogene: Including Oncogene Reviews. 2021; 40:2182-2199.

Petrany, MJ; Swoboda, CO; Sun, C; Chetal, K; Chen, X; Weirauch, MT; Salomonis, N; Millay, DP. Single-nucleus RNA-seq identifies transcriptional heterogeneity in multinucleated skeletal myofibers. Nature Communications. 2020; 11:6374.

Cramer, AA W; Prasad, V; Eftestøl, E; Song, T; Hansson, KA; Dugdale, HF; Sadayappan, S; Ochala, J; Gundersen, K; Millay, DP. Nuclear numbers in syncytial muscle fibers promote size but limit the development of larger myonuclear domains. Nature Communications. 2020; 11:6287.

Hansson, KA; Eftestøl, E; Bruusgaard, JC; Juvkam, I; Cramer, AW; Malthe-Sørenssen, A; Millay, DP; Gundersen, K. Myonuclear content regulates cell size with similar scaling properties in mice and humans. Nature Communications. 2020; 11:6288.

Reid, AL; Wang, Y; Samani, A; Hightower, RM; Lopez, MA; Gilbert, SR; Ianov, L; Crossman, DK; Dell'Italia, LJ; Millay, DP; Van Groen, T; Halade, GV; Alexander, MS. DOCK3 is a dosage-sensitive regulator of skeletal muscle and Duchenne muscular dystrophy-associated pathologies. Human Molecular Genetics. 2020; 29:2855-2871.

Petrany, MJ; Song, T; Sadayappan, S; Millay, DP. Myocyte-derived Myomaker expression is required for regenerative fusion but exacerbates membrane instability in dystrophic myofibers. JCI insight. 2020; 5:136095.

Leikina, E; Melikov, K; Rabinovich, AG; Millay, DP; Chernomordik, LV. Lipid Mixing Assay for Murine Myoblast Fusion and Other Slow Cell-cell Fusion Processes. Bio-protocol. 2020; 10:e3544.