The Pandey lab studies the role of immunoglobulin G isotypes, complement activation products, toll-like receptors, cytokines and chemokines in induction of inflammation in genetic and rare diseases. Using a mouse model and a chemically induced in vivo, ex vivo and in vitro model and human patients with lysosomal storage diseases, Dr. Pandey’s laboratory is currently exploring better understanding of how excess accumulation of certain lipids activate innate and adaptive immune system to trigger visceral and CNS tissue damage.

Additionally, our current research efforts focus on FcγR, C5aR, and TLRs - mediated downstream signaling involved in the inflammatory response. Our laboratory consistently uses some of the standard cellular and immunological techniques such as purification and characterization of several immune cells, (e.g., macrophages, dendritic cells, neutrophils, T and B cell subsets), antigen presentation assay, immunoglobulin isotype switching, ELISA, proteome array, SDS-PAGE, Western blotting, real time PCR, immunohistochemistry, FACS, chemotaxis, protein purification techniques, etc.

Long-Term Research Aims

 

  • Investigate the role of innate and adaptive immune cells for propagation of visceral and central nervous system inflammation in lysosomal storage diseases
  • Uncovering the downstream signaling, that initiate and propagate the immunological inflammation and tissue damage in lysosomal storage and neurodegenerative diseases.