The Ogawa Lab focuses on elucidating non-coding RNAs impact on gene regulation and development in animals using X-chromosome inactivation as a model system.
X-chromosome inactivation, a dosage compensation between XY males and XX females, is a critical process in both extra-embryonic and embryonic development in mammalian females.
Long non-coding RNA Xist has the crucial role of inactivating one of the two X-chromosome during X-chromosome inactivation in female mammals. Mouse female embryonic stem cells are used as a model to recapitulate events occurring during early female development, including X-chromosome inactivation.
We are seeking to understand: (1) how chromosome-wide silencing is induced by Xist RNA, and (2) how long-range silencing is maintained and organized.