The goal of the Cincinnati Center of Excellence in Hematology (CCEH) is to understand and correct at the molecular level diseases affecting cells derived from hematopoietic stem cells.

CCEH facilitates center members to progress toward this goal by fostering interdisciplinary, collaborative approaches, using state-of-the-art molecular and cell biology methods along with timely and rational application of translational studies in the clinical setting.

The Xenotransplant and Genome Editing Core, Gene Delivery Core, and Single Cell Characterization and Procurement Core provide fee-for-service offerings to researchers. The goal of the Enrichment Core is to facilitate scientific exchange and sharing of techniques and resources.

The CCEH is funded by the National Institute of Diabetes and Digestive and Kidney Diseases (NIDDK), and is co-led by Yi Zheng, PhD, and Daniel Starczynowski, PhD.

Xenotransplant and Genome Editing Core

Contact the Co-Directors

Daniel Starczynowski, PhD
daniel.starczynowski@cchmc.org

Damien Reynaud, PhD
damien.reynaud@cchmc.org

Services

  1. Specialized mouse strains to CCEH members in a cost-efficient and timely manner
  2. Irradiation services for animal and cell-based assays
  3. Specialized animal manipulation services that collectively facilitate in vivo analyses of hematopoietic cell functions and assist in drug development for hematological disorders
  4. Technical support and collaborations for comprehensive design and analysis of in vivo studies

Gene Delivery Core

Contact the Co-Directors

Jose Cancelas, MD, PhD
jose.cancelas@cchmc.org

Carolyn Lutzko, PhD
carolyn.lutzko@cchmc.org

Services

  1. Integrating vectors that include (i) γ-retrovirus, (ii) lentivirus and (iii) foamy virus vectors
  2. DNA virus vectors that include AAV and adenovirus vectors 
  3. Access to the broader hematology research community on new technologies on design and production of vectors along with feasibility studies on titers
  4. Process development and scale-up of vectors to support a future GMP production, and to provide pilot medium scale GLP grade vector preparations (produced using the same methodology for the eventual GMP production) for preclinical pharm/tox studies and transduction optimization studies

Single Cell Characterization and Procurement Core

Contact the Co-Directors

Lee Grimes, PhD
lee.grimes@cchmc.org

Marie-Dominique Filippi, PhD
marie-dominique.filippi@cchmc.org

Services

  1. Single-cell sort and analyses by conventional flow cytometry (up to 18 colors), multispectral flow cytometry (up to 24 colors) and flow imaging
  2. Single cell mRNA expression: providing expertise and technical support on study design, sample preparation, and data analyses and interpretation
  3. Super-resolution confocal imaging: providing expertise and technical support on study design, sample preparation and data analyses and interpretation for single cell analysis in vitro and in bone marrow in situ

Enrichment Core

Contact the Co-Directors

Russell Ware, MD, PhD
russell.ware@cchmc.org

Theodosia Kalfa, MD, PhD
theodosia.kalfa@cchmc.org

Services

  1. Weekly Hematology Grand Rounds, Translational Research Retreats, symposia and workshops to promote scientific exchange and communication between clinicians and basic- and translational-research scientists
  2. Mentor two young investigators per cycle as CCEH Research Scholars
  3. Facilitate exchange rotations for post-doctoral fellows and graduate students
  4. Laboratory rotations and summer internships of undergraduate and high school students on non-malignant hematology research